辅助性T淋巴细胞亚群、维生素D在儿童难治性哮喘中的表达研究

Study on expression of helper T lymphocyte subsets and vitamin D in refractory asthma

目的研究辅助性T淋巴细胞亚群、25-(OH)D;水平在难治性哮喘患儿诊疗中的意义。方法选取2013年10月—2020年10月在河南省儿童医院门诊就诊的70例难治性哮喘患儿作为观察组,65例健康儿童作为对照组。分析两组儿童中辅助性T淋巴细胞亚群中Th1、Th2、Th17、Treg的比例,并计算Th1/Th2、Treg/Th17的比值,25-(OH)D;水平的差异。结果难治性哮喘患儿的辅助性T淋巴细胞中Th1细胞比例、Th1/Th2比值、Treg/Th17比值明显低于对照组,Th2、Th17细胞比例高于对照组,差异有统计学意义(P<0.05)。观察组和对照组Treg细胞比例差异无统计学意义(P>0.05)。两组间血清25-(OH)D;水平存在差异,难治性哮喘组血清25羟维生素D;水平低于对照组。结论与正常儿童相比,难治性哮喘患儿存在辅助性T淋巴细胞亚群异常,体内25羟维生素D;水平明显降低。辅助性T淋巴细胞亚群、25羟维生素D;可作为评估难治性哮喘的指标。

Objective To study the significance of helper T lymphocyte subsets and serum 25-(OH)D;level in the diagnosis and treatment of children with refractory asthma.Methods Totally 70 children with refractory asthma and 65 healthy children in the control group were selected,analyzed the proportions of Th1,Th2,Th17 and Treg in helper T lymphocyte subsets,and calculate the ratio of Th1/Th2,Treg/Th17,and the difference of serum 25-(OH)D;level.Results The proportion of Th1 cells,Th1/Th2 ratio and Treg/Th17 ratio in helper T lymphocytes in children with refractory asthma were significantly lower than those in the control group,while the proportion of Th2 and Th17 cells was higher than that in the control group,and the difference was statistically significant(P<0.05).There was no significant difference in the proportion of Treg cells between the observation group and control group(P>0.05).There were differences in serum levels of 25-(OH)D;between the two groups,and the level of 25-(OH)D;in the refractory asthma group was lower than the control group.Conclusion Compared with normal children,children with refractory asthma have abnormal helper T lymphocyte subsets and significantly lower levels of 25-(OH)D;.Helper T lymphocyte subsets and 25-(OH)D;can be used as indicators to evaluate refractory asthma.