温度和盐度对脊尾白虾生长和非特异性免疫的影响

Effects of Temperature and Salinity on the Growth and Non-specific Immune Indices of Exopalaemon carinicauda

为研究高密度养殖条件下温度和盐度对脊尾白虾生长和非特异性免疫功能的影响,本研究设置4个温度梯度(15℃、20℃、25℃、30℃)和4个盐度梯度(15、20、25、30),研究高密度养殖条件下温度和盐度对脊尾白虾特定生长率(SGR)、肥满度(CF)、存活率(SR)、总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、碱性磷酸酶(AKP)、溶菌酶(LZM)等活力的影响。结果显示,脊尾白虾SGR随温度的升高先升高后降低(P<0.05),而随盐度的升高逐渐降低(P<0.05);CF随温度和盐度的变化没有显著性差异(P>0.05);温度对SR影响显著(P<0.05),在30℃时存活率最低,而盐度对SR没有显著影响(P>0.05);T-AOC能力随温度的升高先降低后升高(P<0.05),在20℃时取得最小值,15和30两个盐度组T-AOC能力显著高于20和25盐度组(P<0.05);SOD酶活性在实验温度范围内存在极显著性差异(P<0.01),而盐度对SOD酶活性影响不显著(P>0.05);温度对LZM酶活性影响不显著(P>0.05),15和25盐度组LZM酶活性显著高于20和30盐度组(P<0.05);20℃组AKP酶活性显著高于其他3个温度组(P<0.05),同时20盐度组虾AKP酶活性最高,并显著高于其他盐度组(P<0.05)。

To clarify the effects of temperature and salinity on the growth and non-specific immune indices of Exopalaemon carinicauda under high-density culture conditions,four temperatures(15℃,20℃,25℃,30℃)and four salinities(15,20,25,30)were set in this study.The specific growth rate(SGR),condition factor(CF),survival rate(SR),total antioxidant capacity(T-AOC),superoxide dismutase(SOD),alkaline phosphatase(AKP),lysozyme(LZM)were detected during the experiment.The results showed that SGR tended to increase first and then decrease with the increase of temperature(P<0.05),but tend to increase with the rising of salinity(P<0.05).No significant difference was found among CF of all treatments.SR had shown significant difference among temperatures(P<0.05),of which 30℃was the lowest.However,there was no significant difference among salinities(P>0.05).T-AOC tended to decrease first and then increase with the increase of temperature(P<0.05),of which 20℃was the lowest.T-AOC of salinities 15 and 30 was significantly higher than that of salinities 20 and 25(P<0.05).SOD had shown very significantly different in the temperature group(P<0.01),but no a significant difference among salinities(P>0.05).LZM had shown no significant difference among temperatures(P>0.05).LZM of salinities 15 and 25 was significantly higher than that of salinity 20 and 30(P<0.05).AKP tended to increase first and then decrease with the increase of temperature and salinity(P<0.05),of which 20℃and salinity 20 were the highest.