年龄对非糖尿病捐献者胰岛制备过程和功能的影响

Effects of age on islet preparation and function in non-diabetic donors

目的分析不同年龄捐献者胰腺胰岛制备产量和功能的特点。方法选取2015年6月~2019年12月天津市第一中心医院105例胰腺,均来自非糖尿病器官捐献者,按照<40岁,≥40岁进行分组,其中<40岁组38例,≥40岁组67例。胰岛制备在优良生产规范实验室(good manufacturing practice,GMP)中进行,经过修整,灌注胶原酶,Ricordi消化罐消化,利用连续密度梯度法纯化获得胰岛,检测胰岛产率、活性和葡糖糖刺激的胰岛素分泌能力,评估分离胰岛的数量和质量。结果胰岛纯化前,<40岁组胰岛当量(islet equivalent,IEQ)、IEQ/g较≥40岁组高,差异具有统计学意义(P<0.05),胰岛纯化后<40岁组胰岛个数(islet particle number,IPN)、IPN/g较≥40岁组高,差异具有统计学意义(P<0.01),两组胰岛素分泌差异无统计学意义。结论年轻捐献者可以提高胰岛制备的成功率,非糖尿病年长供者可以获得优质的胰岛。

Objective To investigate the characteristics of pancreas islet production and function in different age donors.Methods From June 2015 to December 2019,a total of 105 cases of pancreases from non-diabetic organ donors were included.They were grouped according to<40 years old and≥40 years old,of whom 38 cases in<40-year-old group and 67 cases in≥40-year-old group.Pancreases were perfused with collagenase,digested in Ricordi digestion tank,and purified through continuous density gradient centrifugation in good manufacturing practice(GMP)laboratory.The islet yield,viability,and glucose-stimulated insulin secretion were measured to evaluate the islet qualities and quantities.Results Before islet purification,the islet equivalent(IEQ)and IEQ/g of the<40-year-old group were significantly higher than those of the≥40-year-old group(P<0.05).After the islet purification,the islet particle number(IPN)and IPN/g of the<40-year-old group were greater than the≥40-year-old group(P<0.01).There was no statistically significant difference in insulin secretion between the two groups.Conclusion Young donors can improve the success rate of islet preparation,and non-diabetic elderly donors can obtain high-quality islets.