色素上皮衍生因子对人脑胶质瘤细胞增殖与凋亡的影响

Effect of pigment epithelial-derived factor on proliferation and apoptosis of human glioma cells

目的探究分析色素上皮衍生因子(pigment epithelium-derived factor,PEDF)对人脑胶质瘤细胞增殖与凋亡的影响。方法培养人胶质母细胞瘤细胞株U87MG,分别添加0 nmol/L、80 nmol/L、160 nmol/L、320 nmol/L PEDF蛋白,记为空白对照组、实验A组、实验B组、实验C组,采用CCK8实验和细胞单克隆实验检测PEDF在U87MG增殖中的作用;采用流式细胞凋亡实验检测PEDF在U87MG凋亡中的作用;采用流式细胞周期实验检测PEDF在U87MG细胞周期中的作用。结果与空白对照组比较,同一时点各实验组细胞增殖率和细胞单克隆能力明显降低(P<0.05),实验C组细胞增殖率与细胞单克隆量明显低于实验A组和实验B组(P<0.05),实验B组的细胞增殖率与单克隆形成量明显低于实验A组(P<0.05);与空白对照组比较,各实验组细胞凋亡率明显增加(P<0.05),细胞G1期、S期进程明显减缓(P<0.05),实验C组细胞凋亡率明显高于实验A、B组(P<0.05),C组S期细胞数明显少于实验A、B组(P<0.05),实验B组细胞凋亡率明显高于实验A组(P<0.05),B组S期细胞数则明显少于实验A组(P<0.05)。结论PEDF能抑制人胶质母细胞瘤细胞U87MG增殖和单克隆形成,并可通过影响细胞周期进程促进U87MG凋亡,其作用与PEDF浓度有关。

Objective To investigate and analyze the effect of pigment epithelial-derived factor(PEDF)on the proliferation and apoptosis of human glioma cells.Methods Human glioblastoma cell lines(U87MG)were cultured with 0 nmol/L,80 nmol/L,160 nmol/L and 320 nmol/L PEDF proteins,which were recorded as blank control group,experimental group A,experimental group B and experimental group C.CCK8 experiment and cell colony formation experiment were used to test the effect of PEDF on U87MG proliferation.The effect of PEDF on U87MG apoptosis was detected by flow cytometry.The effect of PEDF on U87MG cell cycle was detected by flow cytometry.Results Compared with blank control group,the cell proliferation rate and monoclonal capacity of all experimental groups were significantly decreased at the same time point(P<0.05),the cell proliferation rate and monoclonal capacity of experimental group C were significantly lower than those of experimental groups A and B(P<0.05),and the cell proliferation rate and monoclonal capacity of experimental group B were significantly lower than those of experimental group A(P<0.05).Compared with the blank control group,the apoptosis rate of all experimental groups was significantly increased(P<0.05),the process of G1 phase and S phase was significantly slowed down(P<0.05),the apoptosis rate of experimental group C was significantly higher than that of experimental group A and experimental group B(P<0.05),and the cell number of C phase and S phase was significantly lower than that of experimental group A and experimental group B(P<0.05).The apoptosis rate of experimental group B was significantly higher than that of experimental group A(P<0.05),and the number of cells in B and S stages was significantly lower than that in experimental group A(P<0.05).Conclusion PEDF can inhibit the proliferation and colony formation of human glioblastoma cells U87MG,and promote the apoptosis of U87MG by affecting the cell cycle process.Its effect is also dependent on the concentration of PEDF.