痛泻要方联合双歧杆菌三联活菌对IBS模型大鼠肠道动力及SCF/C-Kit信号通路的影响

Effects of Tongxie decoction combined with bifidobacterium triple bacteria on intestinal dynamics and SCF/C-Kit signaling pathway in IBS model rats

目的探讨痛泻要方联合双歧杆菌三联活菌对肠易激综合征(irritable bowel syndrome,IBS)大鼠结肠动力及干细胞因子(stem cell factor,SCF)/酪氨酸激酶受体(C-Kit)信号通路的影响。方法将150只清洁级雄性Wistar大鼠进行IBS造模,随机分为对照组、模型组、痛泻要方组、双歧杆菌三联活菌组、联合用药组,每组30只。观察比较大鼠体重变化、直肠玻璃小球排出时间、小肠推进率,酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)血浆中C-Kit、SCF的表达,Western blot检测结肠组织中C-Kit、SCF的表达。结果与模型组比较,双歧杆菌三联活菌组、痛泻要方组、联合用药组的玻璃小球排出时间延长(P<0.05),小肠推进率降低(P<0.05),血清及结肠组织中C-Kit、SCF的表达降低(P<0.05),且联合用药组的疗效更为显著。结论痛泻要方联合双歧杆菌三联活菌有效改善了IBS模型大鼠结肠的转运功能及小肠推进率,效果优于单独用药,其机制可能是通过调控SCF/C-Kit信号通路进而调节肠道的运动。

Objective To investigate the effects of Tongxie decoction combined with bifidobacterium triple bacteria on colonic dynamics and stem cell factor(SCF)/C-Kit signaling pathways in irritable bowel syndrome(IBS)rats.Methods The model of IBS 150 rats clean Wistar rats were randomly divided into control group,model group,Tongxie decoction group,bifidobacterium triple bacteria group and combined drug group,with 30 rats in each group.Rats weight,the expulsion time of glass spheres in the rectum,small intestinal propulsion rate were observed and compared;The expression of C-Kit,SCF in plasma were detected by enzyme-linked immunosorbent assay(ELISA);The expression of C-Kit,SCF in colon tissue were detected by Western blot.Results Compared with the model group,the expulsion time of glass spheres were prolonged in the bifidobacterium triple bacteria group,the Tongxie decoction group,and the combined drug group(P<0.05),small intestinal propulsion rate was decreased(P<0.05),and expression of C-Kit and SCF in serum and colonic tissues was decreased(P<0.05).The efficacy of the combined drug group was more significant(P<0.05).Conclusion The combination of Tongxie decoction and bifidobacterium triple bacteria can effectively improve the transit function and small intestinal propulsion rate of the colon,and the effect was superior to that of the drug alone.The mechanism may be through the modulation of the SCF/C-Kit signaling pathway and thus the regulation of intestinal motility.