甲基丙烯酸酐化明胶/海藻酸钠纳米复合水凝胶对小鼠前成骨细胞增殖分化的影响

Effect of gelatin methacrylate/sodium alginate nanocomposite hydrogel on the proliferation and differentiation of MC3T3-E1 cells

目的制备甲基丙烯酸酐化明胶(gelatin methacrylate,GelMA)、海藻酸钠(sodium alginate,Alg)及纳米羟基磷灰石(nano-hydroxyapatite,nHA)复合水凝胶材料(GelMA-Alg-nHA),对其理化性能、细胞相容性及成骨分化作用进行研究。方法通过紫外光交联制备GelMA-Alg-nHA复合水凝胶。将实验材料分为GelMA、GelMA-nHA、GelMA-Alg和GelMA-Alg-nHA 4组,通过扫描电子显微镜、力学测试对其进行结构表征和性能测试。通过体外细胞实验检测4组水凝胶对小鼠前成骨细胞MC3T3-E1细胞活性、黏附、增殖及碱性磷酸酶(alkaline phosphatase,ALP)活性的影响。结果各组水凝胶均具有多孔结构,GelMA-Alg-nHA水凝胶较其余几组水凝胶孔径略小,孔洞表面更粗糙,材料强度与GelMA比较从(9.75±1.27)kPa提高到(30.02±2.41)kPa(P<0.001)。体外实验显示,GelMA-Alg-nHA水凝胶具有良好的生物相容性,促进成骨细胞的增殖分化的能力更强(P<0.001)。结论GelMA-Alg-nHA水凝胶具有良好的生物相容性和诱导MC3T3-E1成骨分化的能力,可成为潜在的骨缺损修复材料。

Objective To prepare gelatin methacrylate(GelMA),sodium alginate(Alg)and nano-hydroxyapatite(nHA)hydrogel materials,and to study the physicochemical properties,cell compatibility and effect of GelMA-Alg-nHA on osteogenic differentiation.Methods GelMA-Alg-nHA hydrogel was prepared by UV cross-linking.The experimental materials were divided into 4 groups:GelMA group,GelMA-nHA group,GelMA-Alg group,and GelMA-Alg-nHA group,and their structural characterization and performance were tested by scanning electron microscopy and mechanical testing.The effects of four groups of hydrogels on MC3 T3-E1 cell activity,adhesion,proliferation,and alkaline phosphatase(ALP)activity were determined by in vitro cell assays.Results All groups of hydrogel had porous structures,and the GelMA-Alg-nHA hydrogel had slightly smaller pore sizes and rougher pore surfaces compared with the remaining groups of hydrogel,its material strength increased from(9.75±1.27)kPa to(30.02±2.41)kPa(P<0.001)compared with that of GelMA.In vitro experiments showed that the GelMA-Alg-nHA hydrogel had good biocompatibility and a stronger ability to promote the proliferation and differentiation of osteoblasts(P<0.001).Conclusion The GelMA-Alg-nHA hydrogel has good biocompatibility and the ability to induce osteogenic differentiation of MC3 T3-E1,may be a potential material for bone defect repair.