碳点-金纳米簇结合的双发射荧光探针的制备及传感应用

Preparation and Application of Dual-Emission Fluorescent Probe Based on Carbon Dots Coupled with Gold Nanoclusters

先以柠檬酸为原料采用水热合成法合成了碳点(CD),再以乙酰化含硫超支化聚酰胺-胺[HPA(S)-Ac]为模板和还原剂还原氯金酸(HAuCl4)制备了金纳米簇(Au NCs),碳点和金纳米簇溶液混合后得到双发射荧光探针(CD-AuNCs),透射电镜结果表明CD和AuNCs粒径均接近2 nm,在CDAu NCs中分散均匀。考察p H值对CD-AuNCs荧光性能的影响时发现CD-AuNCs的荧光在pH=6达到最强,且在此pH值下CD-AuNCs对四环素类药物(TCs)的响应灵敏,能够实现对TCs的传感。CDAu NCs探针不仅选择性好,而且和单一CD探针相比具有线性范围更宽、灵敏度更高的优点。

Carbon dots(CD)were synthesized from citric acid using a hydrothermal method,and gold nanoclusters(AuNCs)were prepared from chloroauric acid(HAuCl4)with sulfur-containing hyperbranched polyamide-amines as template and reducing agent.Then,the double-emitting fluorescent probes(CD-AuNCs)were obtained by mixing carbon dots and gold nanoclusters.Transmission electron microscopy images showed that the particle diameters of CD both were about 2 nm and dispersed uniformly.The effect of pH on the fluorescence performance of CD-AuNCs was investigated.It was found that the fluorescence of CD-AuNCs reached the strongest at pH=6,and CD-AuNCs responsed sensitively to tetracyclines(TCs)at this pH.The CD-AuNCs probe is not only highly selective,but also has the advantages of a wider linear range and a higher sensitivity than the single CD probe.