摘要
Au@Fe3O4作为固定化载体,以亲和素-生物素的特异性结合对氯过氧化物酶进行固定化,并对固定化酶的重复使用性进行了研究。结果表明,Au@Fe3O4载体是以Fe3O4为核Au为壳的核-壳型纳米材料,磁性分离效果较好,在酸性介质中较为稳定。固定化温度10℃、pH值为3.0、固定化时间40min,氯过氧化物酶的固载量相对较高。固定化酶在重复使用6次仍保留70%以上的酶残余活性,可以实现多次重复使用。
Chloroperoxidase was immobilized with the carrier of Au@Fe3O4 by the method of specific binding of avidin-biotin,and the reusability of immobilized enzyme was studied.The results showed that the Au@Fe3O4 carrier was a core-shell nanoparticles with Fe3O4 as the core and Au as the shell.The magnetic separation effect of Au@Fe3O4 was good and stable in acid medium.With the temperature of 10℃,the pH 3.0,and the time of 40 min,the immobilized amount of chloroperoxidase was relatively high.After being reused for 6 times,the residual activity of immobilized enzyme was still more than 70%,which could be reused for many times.
作者
高丰琴
陈晓丽
郭向宇
牟萱
GAO Feng-qin;CHEN Xiao-li;GUO Xiang-yu;MOU Xuan(College of Chemistry and Chemical Engineering,Xianyang Normal University,Xianyang 712000,China;School of Chemistry&Chemical Engineering,Shaanxi Normal University,Xi'an 710119,China)
出处
《化学工程师》
CAS
2020年第12期11-13,5,共4页
Chemical Engineer
基金
陕西省教育厅科研计划项目资助(No.16JK1827)
咸阳师范学院(国家级)大学生创新创业训练计划项目(S202010722011)
