摘要
目的:探讨白藜芦醇后处理对脑缺血再灌注大鼠血脑屏障及ICAM-1、VCAM-1的影响。方法:随机将105只SD大鼠分为:假手术组,I/R组,Res组:低剂量组(10mg/kg/d)、中剂量组(20mg/kg/d)、高剂量组(40mg/kg/d)。线栓法制备大鼠MCAO模型,I/R后24h,观察大鼠神经功能评分,伊文思蓝法观察大鼠BBB通透性,干湿重法观察大鼠脑水肿,电镜观察大鼠BBB超微结构,ELISA法观察脑组织中ICAM-1、VCAM-1的表达。结果:高剂量组大鼠神经功能评分低于I/R组;I/R组脑组织中依文思蓝的含量高于假手术组,Res后处理组依文思蓝含量低于I/R组,Res高剂量组与I/R组比较有统计学意义;I/R组脑水肿程度高于假手术组,Res组脑水肿程度低于I/R组,Res高剂量组与I/R组比较有统计学意义;白藜芦醇组可以改善大鼠BBB超微结构,高剂量组更为显著;I/R组ICAM-1和VCAM-1的水平高于假手术组,Res组ICAM-1和VCAM-1的水平低于I/R组,Res高剂量组与I/R组比较有统计学意义。结论:白藜芦醇可能通过下调ICAM-1和VCAM-1,改善大鼠BBB通透性,改善BBB超微结构,减轻I/R后脑水肿程度,且具有剂量依赖性。
Objective To investigate the effects of post-treatment of resveratrol on BBB and the level of ICAM-1、VCAM-1 after cerebral I/R.Methods 105 SD rats were randomly divided into:sham group,I/R group,Res low-dose group,medium-dose group,high-dose group.MCAO model were prepared by the suture method.After 24h of I/R,the neurological function scores were observed.The permeability of BBB were observed by Evans blue method.Brain edema were observe by dry and wet weights,electron microscope to observe the ultrastructure of BBB,ELISA method to observe the expression of ICAM-1 and VCAM-1.Results The neurological scores in the high-dose group were lower than those in I/R group.The content of Evans blue in I/R group were higher than that in sham group,those in the Res groups were lower than that in the I/R group.Cerebral edema in I/R group were higher than that in sham group,those in Res group were lower than that in I/R group,high-dose were significant.Res groups can improve the ultrastructure of BBB,especially in the high-dose.The levels of ICAM-1 and VCAM-1 in I/R group were higher than those in sham group,the levels of ICAM-1 and VCAM-1 in Res groups were lower than those in I/R group,high-dose were significant.Conclusion Resveratrol may decrease the permeability of the BBB by down-regulating ICAM-1 and VCAM-1,improve the degree of ultrastructure of BBB,thereby reduce the degree of brain edema,and it is dose-dependent.
作者
张晋霞
张志勇
张蕊
刘斌
费云瀚
毛文静
邓春颖
李世英
Zhang Jin-xia;Zhang Zhi-yong;Zhang Rui;Liu Bin;Fei Yun-han;Mao Wen-jing;Deng Chun-ying;Li Shi-ying(North China University of Science And Technology Affiliated Hospital,Tangshan 063000,China)
出处
《湖南师范大学学报(医学版)》
2021年第6期39-41,共3页
Journal of Hunan Normal University(Medical Sciences)
基金
河北省教育厅科研基金项目(NO.QN2017119)
2017年河北省政府资助省级临床医学优秀人才课题(题目:白藜芦醇对脑缺血再灌注大鼠的保护作用及机制研究)
河北省中医药类科研课题(NO.2017205)