乙酰水杨酸应用于牙龈干细胞对免疫相关疾病的调节功能及机制研究

Study of acetylsalicylic acid used in mesenchymal stem cells derived from gingiva for immune related diseases on the regulating function and mechanism

目的:探究乙酰水杨酸(ASA)应用于牙龈干细胞(GMSCs)对实验性自身免疫性脑脊髓炎(EAE)的作用及机制。方法:建立GMSCs与T细胞体外共培养系统,通过流式分析、MTT实验检测ASA对T细胞凋亡、增殖的影响。MTT实验检测ASA对GMSCs增殖能力的影响,流式分析ASA对GMSCsTreg和Th17细胞分化的影响。制作小鼠EAE模型,研究GMSCs对EAE的治疗作用,并分析ASA对GMSCs免疫调节功能的促进作用。结果:GMSCs诱导T细胞凋亡,同时抑制T细胞增殖。ASA可以促进GMSCs诱导T细胞凋亡、抑制T细胞增殖的作用。ASA可以显著提高GMSCs的细胞增殖率。GMSCs能够促进Treg细胞并抑制Th17细胞分化,ASA能够增强GMSCs对T细胞的调节分化作用。注射GMSCs对EAE小鼠具有治疗作用,ASA可以促进GMSCs对EAE小鼠的治疗疗效。结论:ASA可以增强GMSCs的免疫调节功能,增强GMSCs对EAE小鼠的治疗作用。

Objective To investigate the effect and mechanism of acetylsalicylic acid(ASA)on experimental autoimmune encephalomyelitis(EAE).Methods A co-culture system of GMSCs and T cells in vitro was established,and the effects of ASA on apoptosis and proliferation of T cells were detected by flow cytometry and MTT experiments.The effect of ASA on the proliferation of GMSCs was detected by MTT assay,and the effect of ASA on the differentiation of GMSCsTreg and Th17 cells was analyzed by flow cytometry.A mouse EAE model was established to study the therapeutic effect of GMSCs on EAE,and to analyze the role of ASA in promoting the immune regulation of GMSCs.Results GMSCs induced T cell apoptosis and inhibited T cell proliferation.ASA can promote GMSCs to induce T cell apoptosis and inhibit T cell proliferation.ASA can significantly increase the cell proliferation rate of GMSCs.GMSCs can promote Treg cells and inhibit Th17 cell differentiation,and ASA can enhance the regulation and differentiation of T cells by GMSCs.GMSCs injection has therapeutic effect on EAE mice,ASA can promote the therapeutic effect of GMSCs on EAE mice.Conclusion ASA can enhance the immunoregulatory function of GMSCs and enhance the therapeutic effect of GMSCs on EAE mice.