河南省一起伤寒疫情的病原特征及其分子分型

Pathogenic characteristics and molecular typing of a typhoid epidemic in Henan province

目的了解2018年河南省一起伤寒疫情的病原特征及其分子分型特征。方法对伤寒疫情中所分离的26株菌株进行生化鉴定,并开展药物敏感试验(antimicrobial susceptibility testing,AST)及脉冲场凝胶电泳(pulsed field gel electrophoresis,PFGE)分型。结果26株分离株均为伤寒沙门菌,血清抗原式为9,12:d:-。药敏结果显示,26株菌株除了磺胺异噁唑的耐药率为3.85%、链霉素的耐药率为7.69%外,对其他有抗生素都敏感。PFGE结果显示,分离的26株伤寒沙门菌经XbaI酶切后为同一种带型,菌株来源病例具有高度聚集性。结论本次暴发疫情是由同一遗传克隆的伤寒沙门菌传播而导致。

Objective To understand the pathogenic characteristics and molecular typing of a typhoid epidemic in Henan in 2018.Methods Biochemical identification,antimicrobial susceptibility testing(AST)and pulsed field gel electrophoresis(PFGE)of 26 strains isolated from typhoid fever were conducted.Results All the 26 isolates were Salmonella typhi,and the serum antigen formula was 9,12:d:-.The results of AST showed that all antibiotics were sensitive except sulfaisoxazole(3.85%)and streptomycin(7.69%).PFGE results showed that 26 strains of Salmonella typhi isolated were divided into one band type after XbaI digestion,and the strains were highly clustered.Conclusion The outbreak was caused by the transmission of the same genetic clone of Salmonella typhi.