三重实时荧光PCR检测方法在病毒性腹泻病原体检测中的应用

Application of triple real-time fluorescent PCR in the detection of viral diarrhea pathogens

目的了解腹泻样本中病毒性腹泻病原体情况,为本地区病毒性腹泻疾病的防控提供基础资料。方法收集2019年本院的61份临床腹泻病人粪便标本,提取核酸,用三重实时荧光PCR检测方法进行检测。结果在61份临床腹泻标本中检测出轮状病毒阳性标本3份,腺病毒阳性标本1份,诺如病毒GI型标本1份,诺如病毒GII型标本7份。结论腹泻样本中有多种致腹泻病毒存在,病毒性腹泻病原组成较为复杂,建议开展诺如病毒、轮状病毒、腺病毒的分子流行病学研究。

Objective To understand the pathogen of viral diarrhea in diarrhea samples and provide basic data for the prevention and control of viral diarrhea in the studied area.Methods In 2019,61 stool samples of diarrhea patients in Zhengzhou People’s Hospital were collected,and nucleic acid was extracted and detected by triple real-time fluorescent PCR.Results Among 61 clinical diarrhea samples,3 were positive for rotavirus,1 for adenovirus,1 for norovirus GI and 7 for norovirus GII.Conclusion There are many kinds of diarrheal viruses in diarrhea samples,and the pathogen composition of viral diarrhea is complex.It is suggested to carry out the molecular epidemiological study on norovirus,rotavirus and adenovirus.