摘要
为了探讨稳定同位素碳、氮、氧、氢对羊肉产地溯源的可行性,本研究分别从内蒙古、新疆、宁夏、新西兰、意大利5个地区采集羊肉样品,利用稳定同位素比率质谱仪(IRMS)测定脱脂羊肉的碳、氮、氧、氢稳定同位素比值(δ^(13)C、δ^(15)N、δ^(2)H、δ^(18)O),结合多元统计学方法对不同地区间脱脂羊肉的δ^(13)C、δ^(15)N、δ^(2)H、δ^(18)O值组成特征及变化规律进行分析。结果表明,δ^(13)C值在5个地区间均存在显著性差异,而δ^(15)N值和δ^(18)O值除了在新西兰和意大利2个地区差异不显著外,在其他地区差异均显著;δ^(2)H值仅在宁夏和新疆地区间以及内蒙古和新西兰地区间差异不显著。结合Fisher判别分析发现,利用δ^(13)C、δ^(15)N、δ^(2)H、δ^(18)O值可以实现对羊肉产地区分,且利用δ^(13)C、δ^(15)N、δ^(2)H、δ^(18)O值组合指标对羊肉产地的初始判别的整体正确率和交叉验证的整体判别正确率均达100%。本研究结果为δ^(13)C、δ^(15)N、δ^(2)H、δ^(18)O值在羊肉产地溯源中的应用提供了参考。
In order to explore the feasibility of using stable isotope to trace the geographical origin of mutton,mutton muscle samples were collected from Inner Mongolia,Xinjiang,Ningxia,New Zealand and Italy.The carbon,nitrogen hydrogen and oxygen stable isotope ratio of de-fatted mutton muscle were analyzed using isotope ratio mass spectrometry(IRMS).Significant differences were observed inδ^(13)C value of de-fatted meat samples from different regions using multivariate analysis of variance analysis.Theδ^(15)N value andδ^(18)O value of de-fatted meat samples were also significantly different,except for the samples from New Zealand and Italy.The δ^(2)H value was not unremarkable in Ningxia and Xinjiang,and for mutton from Inner Mongolia and New Zealand.Based on stable isotope values and Fisher discriminant analysis,it could be concluded thatδ^(13)C、δ^(15)N、δ^(2)H、δ^(18)O values can be used to distinguish the origin of mutton,and the initial discrimination rate and cross-validated discrimination rate of classification can be achieved by 100%,which provided a reference for the application ofδ^(13)C、δ^(15)N、δ^(2)H、δ^(18)O values in the origin traceability of mutton.
作者
米瑞芳
陈曦
戚彪
熊苏玥
李家鹏
乔晓玲
王尚轩
MI Ruifang;CHEN Xi;QI Biao;XIONG Suyue;LI Jiapeng;QIAO Xiaoling;WANG Shangxuan(Beijing Key Laboratory of Meat Processing Technology China Meat Research Center,Beijing 100068)
出处
《核农学报》
CAS
CSCD
北大核心
2020年第S01期89-95,共7页
Journal of Nuclear Agricultural Sciences
基金
宁夏回族自治区东西部科技合作计划项目(2017BY068)