稳定同位素技术鉴别库尔勒香梨产地可行性研究

Geographical Origins Identification of Korla Fragrant Pear Based on Stable Isotope Ratio

为开展碳、氮、氢、氧(C、H、O、N)稳定同位素鉴别库尔勒香梨产地的可行性研究,明确新疆库尔勒香梨δ^(13)C、δ^(15)N、δ^(2)H、δ^(18)O值,本研究以甘肃香梨和陕西红香酥梨作为对照,采用稳定同位素比值质谱仪(IRMS)分析新疆库尔勒香梨与对照样品的δ^(13)C、δ^(15)N、δ^(2)H、δ^(18)O值,并采用正交校正的偏最小二乘判别分析(OPLS-DA)鉴别新疆库尔勒香梨产地真实性。结果表明,新疆、甘肃两个产区香梨与陕西产区红香酥梨的δ^(13)C、δ^(15)N、δ^(2)H、δ^(18)O值存在显著差异(P<0.05)。新疆产区的66份库尔勒香梨判别准确率为95.45%;甘肃产区的20份香梨样品判别准确率为60%;陕西产区的23份红香酥梨样品判别准确率为95.65%。C、N、H、O 4种稳定同位素可有效鉴别新疆库尔勒香梨和陕西红香酥梨,但在新疆库尔勒香梨与甘肃香梨鉴别方面,误判率较高,如果能结合其他鉴别技术,则有可能出现较好的鉴别效果。本研究为库尔勒香梨产地真实性鉴别提供了科学依据。

The purpose of this study is to analyze theδ^(13)C、δ^(15)N、δ^(2)H、δ^(18)O isotopic ratio characteristic value of Xinjiang Korla Fragrant Pear(KFP),and to explore the feasibility of identifying the geographical origins of KFP by stable C,N,H,O isotope.Shaanxi Hongxiangsu pear and Gansu Fragrant pear were used as control samples,theδ^(13)C、δ^(15)N、δ^(2)H、δ^(18)O value of KFP and control samples were analyzed by isotope ratio mass spectrometry(IRMS),and the orthogonal correction partial least squares discriminant analysis(OPLS-DA)was used to identify the authenticity of KFP.There were significant differences in theδ^(13)C、δ^(15)N、δ^(2)H、δ^(18)O value among Xinjiang,Gansu and Shaanxi Province(P<0.05).The accuracy rate of KFP in Xinjiang were 95.45%,while those of Hongxiangsu pear in Shaanxi Province were 95.65%.However,the accuracy rate of Gansu Province was low,which was 60%.In conclusion,C,N,H and O can effectively identify Xinjiang KFP and Shaanxi Hongxiangsu pear,but the misjudgment rate is high to discriminate Xinjiang KFP and Gansu fragrant pear.Better identification results might be obtained when combined with other identification techniques.