大麦籽粒总花色苷含量的遗传变异及其与粒色的相关分析

Genetic Variation of Total Anthocyanin Content in Barley Grains and Its Correlation With Grain Color

有色大麦较普通大麦含有较多花色苷等活性成分,具有较好的保健和辅助治疗功能。为了解大麦籽粒花色苷的遗传特点,选育高花色苷含量的大麦新品系,本研究以紫光芒裸二棱×Schooner构建的193个重组自交系为材料,测定3个试点[玉溪(2013年)、白邑(2014年)及嵩明(2015年)]的大麦籽粒总花色苷含量和粒色,分析大麦籽粒总花色苷含量的遗传变异及其与粒色的相关性。结果表明,大麦RIL群体籽粒总花色苷含量遗传变异大,3个试验点大麦总花色苷含量变异范围分别为0.36~1.38、0.50~1.50、0.50~1.58 mg·g-1,变异系数为25.00%~33.85%。大麦总花色苷含量呈右偏态、尖顶峰分布,由主效基因控制遗传。高海拔冷凉气候在一定程度上有利于大麦籽粒总花色苷含量累积。3个试点大麦总花色苷含量与粒色均呈极显著正相关,表明大麦籽粒颜色越深,其总花色苷含量越高。与紫光芒裸二棱相比,3个试点有8个共同株系(27、34、35、37、38、60、138、167)的总花色苷含量显著或极显著提高。本研究结果为高花色苷含量大麦种质创新及育种提供了一定的理论依据。

As compared to common barley,colored barley has multiple benefits and therapeutic functions owing to its accumulation of active ingredients like anthocyanin.To explore inheritance characteristics of anthocyanin and breeding of new barley lines with high anthocyanin content.193 RIL populations,derived from the cross between Ziguangmangluoerleng(purple but high value barley parent)and Schooner(yellow but low value barley parent),were grown at three different sites of Yunnan Province of China for three consecutive years(2013-2015).Genetic variability of total anthocyanin content and its correlation with grain color level was investigated.Results revealed that there were wide genetic variations in total anthocyanin content of barley RIL population.Total anthocyanin content at three-sites(Yuxi-2013,Baiyi-2014 and Songming-2015)ranged from 0.36 to 1.38 mg·g-1,0.50 to 1.50 mg·g-1,and 0.50 to 1.58 mg·g-1,respectively and variation coefficient ranged from 25.00%to 33.85%.The total anthocyanin content of barley RIL population manifested a right skewed and sharp peak distribution,controlled by the major genes.High altitude and low temperature may promote the accumulation of the anthocyanin in barley grains.Total anthocyanin content manifested significant positive correlation with grain color at all three sites.To a certain extent,the deeper the color of barley grain,the higher the content of total anthocyanin content.Compared with Ziguangmangluoerleng(high value parent),total anthocyanin content of 8 common lines at all the three sites increased significantly,which were 27,34,35,37,38,60,138 and 167,respectively.This study provides a theoretical basis for germplasm innovation and breeding of high anthocyanin barley.