大白菜抽薹相关基因BrFLC1的KASP标记开发

Development of KASP Marker for Bolting Related Gene BrFLC1 in Chinese Cabbage(Brassica rapa L. ssp. pekinensis)

为了提高大白菜耐抽薹品种的分子育种效率,本研究针对大白菜抽薹相关基因BrFLC1第6个内含子的第一个碱基(Pi6+1)G-A的SNP变异开发进行高通量检测的竞争性等位基因特异性PCR(KASP)标记。结果表明,开发的KASP标记BrFLC1-KASP1可有效将晚抽薹类型材料Y177-12(G)和早抽薹类型材料Y195-93(A)分为2组。利用BrFLC1-KASP1标记可以对57份大白菜材料的基因型进行有效鉴别,且鉴定结果与酶切扩增多态性标记(CAPS)G-MvaI以及直接测序法的鉴定结果完全一致。综上所述,BrFLC1-KASP1标记在大白菜材料中具有通用性,且具有准确率高、成本低、效率高的特点。本研究结果对大白菜耐抽薹性的分子标记辅助选择具有重要的育种实践价值。

In order to improve the efficiency of molecular marker assisted breeding for bolting-resistant varieties in Chinese cabbage(Brassica rapa L.ssp.pekinensis),we dedicated to develop a KASP(Kompetitive allele specific PCR)marker for high-throughput detection of the G-A variation at the first base in intron 6(Pi6+1)of gene BrFLC1,which is significantly associated with bolting time phenotype.It turned out that the developed KASP marker BrFLC1-KASP1 can successfully differentiate the late-bolting genotype Y177-12(G)from the early-bolting genotype Y195-93(A).The genotypes of BrFLC1 gene in 57 accessions were successfully identified by marker BrFLC1-KASP1.In addition,the genotypes of 57 accessions identified by marker BrFLC1-KASP1 were all same with that identified by CAPS(Cleaved Amplified Polymorphic Sequence)marker G-MvaI and direct sequencing,which suggested that this marker is possessed of stronger applicability and generality in Chinese cabbage,furthermore,it is much more economic and efficient.Thus,the marker BrFLC1-KASP1 developed in this study can be used for marker-assisted selection in bolting-resistance breeding with lower cost and higher efficiency,which has pivotal significance in breeding practice.