多环芳烃内暴露对于血脂的影响以及氧化应激途径的中介作用——一项缺血性脑卒中的病例对照研究

Effects of internal exposure to polycyclic aromatic hydrocarbons on lipids and mediation of oxidative stress pathways:a case-control study of ischemic stroke

目的研究多环芳烃内暴露与血脂之间的关系以及氧化应激途径在其中的中介作用。方法于2021年11月至2023年6月,在天津市西青医院和杨柳青社区医院招募缺血性脑卒中病人和对照人群,171名病例与342名对照人群作为研究对象。测定尿中多环芳烃代谢物、氧化应激指标8-羟基-2’-脱氧鸟苷(8-OHdG)和3-硝基酪氨酸(3-NT)。采用多元线性回归分析多环芳烃内暴露与血脂以及氧化应激指标的关联,采用中介效应分析氧化应激途径在多环芳烃内暴露对血脂改变的中介作用。结果研究总人群总多环芳烃羟基代谢物(ΣOH-PAHs)为187.02μg/g Cr。与对照组相比,缺血性脑卒中组的ΣOH-PAHs水平更高(P<0.05)。ΣOH-Flu和ΣOH-PAHs与低密度脂蛋白与高密度脂蛋白的比值(LDL/HDL)呈正向关联(P<0.05)。ΣOH-Flu与8-OHdG呈正向关联(P<0.05),ΣOH-Nap、ΣOH-Flu、ΣOH-Phe和ΣOHPAHs与3-NT呈正向关联(P<0.05)。8-OHdG在ΣOH-Flu和ΣOH-PAHs与LDL/HDL的关联之间存在中介作用,中介比例为12.00%和8.20%(P<0.05)。3-NT在ΣOH-Flu和ΣOH-PAHs与LDL/HDL的关联之间存在中介作用,中介比例为26.74%和25.76%(P<0.05)。结论多环芳烃内暴露与血脂之间呈显著相关性,氧化应激途径在多环芳烃内暴露与血脂关联中存在中介作用。

Objective To understand the relationship between internal exposure to polycyclic aromatic hydrocarbons and blood lipids and the mediating role of oxidative stress pathways therein.Methods From November 2021 to June 2023,the patients with ischemic stroke and the control population were recruited at Xiqing Hospital and Yangliuqing Community Hospital in Tianjin,China,and 171 cases and 342 control population served as the study subjects.Urinary PAH metabolites,oxidative stress indicators 8-hydroxy-2'-deoxyguanosine(8-OHdG)and 3-nitrotyrosine(3-NT)were measured.Multiple linear regression was used to analyze the association between internal PAH exposure and blood lipids as well as oxidative stress indicators,and the mediating effect was used to analyze the mediating role of oxidative stress pathway in the alteration of blood lipids by internal PAH exposure.Results Total polycyclic aromatic hydrocarbon hydroxyl metabolites(ΣOH-PAHs)in the total study population was 187.02μg/g Cr.ΣOH-PAHs levels were higher in the ischemic stroke group compared to the control group(P<0.001).ΣOH-Flu andΣOH-PAHs were positively correlated with the ratio of low-density lipoproteins to highdensity lipoproteins(LDL/HDL)(P<0.05).ΣOH-Flu was positively correlated with 8-OHdG.positive association(P<0.05),andΣOH-Nap,ΣOH-Flu,ΣOH-Phe,andΣOH-PAHs were positively associated with 3-NT(P<0.05).8-OHdG mediated between the association ofΣOH-Flu andΣOH-PAHs with LDL/HDL,with mediation ratios of 12.00%and 8.20%(P<0.05).3-NT was positively associated with the ratio of LDL/HDL in the association ofΣOH-Flu andΣOH-PAHs There was a mediating role between the association with LDL/HDL with mediating proportions of 26.74%and 25.76%(P<0.05).Conclusion There is a significant correlation between internal PAH exposure and lipids,and the oxidative stress pathway may mediate the association between internal PAH exposure and lipids.