基于细菌SOS反应的水质遗传毒性测试方法的研究进展

Water genotoxicity test methods based on SOS reaction of bacteria:a review of recent studies

基于分子生物学技术和细菌SOS修复反应建立的SOS/umu试验法,具有操作便利、检测敏感性高、测试费用低等优点,成为检测环境致突变物和致癌物的短期试验中应用最广泛的方法之一。笔者对基于细菌SOS反应的遗传毒性测试方法的设计原理、研究进展及其在水质遗传毒性监测的应用等方面做了系统综述,并对SOS/umu试验法的进一步推广提出了建议和展望。

SOS/umu test based on molecular biological technology and bacterial SOS repair reaction has the advantages of convenient operation,high sensitivity and low cost.It has become one of the most widely used methods in environmental mutagens and carcinogens short-term test.In this paper,the design principle,research progress and application of the test method based on bacterial SOS reaction in water genotoxicity monitoring were reviewed systematically,and the suggestions and prospects for further popularization of SOS/umu test were put forward also.