缺氧及厌氧环境下厌氧氨氧化菌的衰减系数

Determination of decay coefficients of Anammox bacteria under anoxic and anaerobic conditions

衰减系数是表征微生物增长的重要动力学参数,与所处环境密切相关。采用基质利用速率测定方法,以厌氧氨氧化工艺中最常见的厌氧氨氧化菌(Candidatus Brocadia)为对象,探讨了其在缺氧(NO2--N、NO3--N)及厌氧环境下的衰减系数。结果表明:基质利用速率测定方法可有效避免由于其他细菌的衰减而引起的实验误差;缺氧(NO2--N、NO3--N)及厌氧环境下厌氧氨氧化菌的衰减系数依次为0.035 2 d^-1、0.025 7 d^-1和0.051 2 d^-1,相比于其他自养菌,厌氧氨氧化菌的衰减系数较小。在进行污泥保存时,维持NO3--N的缺氧环境有利于厌氧氨氧化菌活性和数量的保存。

As an important parameter to characterize the growth of microorganism, decay coefficient is closely related to the environment conditions. In this study, the decay coefficient of the most common anammox bacteria(Candidatus Brocadia) was analyzed by substrate utilization rate determination method under anoxic( NO2--N, NO3--N) and anaerobic conditions. The results showed that substrate utilization rate determination method could effectively avoid the experimental errors caused by the decay of other bacteria. The decay coefficients of anammox bacteria under anoxic(NO2--N, NO3--N) and anaerobic conditions were 0.035 2, 0.025 7 and 0.051 2 d^-1, respectively. Compared with other autotrophic bacteria, it was observed that decay coefficient of anammox bacteria was smaller. An anoxic condition of NO3--N was favorable to the preservation of activity and quantity of anammox bacteria during sludge storage.