利用近红外长余辉发光成像技术研究纳米颗粒经肺暴露后在生物体的迁移途径

A near-infrared long-term persistent luminescence imaging technique for tracking nanoparticles after endotracheal instillation

大气中的超细颗粒物(粒径小于100 nm)由于其超小的粒径,容易进入生物体的循环系统,对其造成极大的伤害;并且因其粒径小,这些超细颗粒物很难在生物体内被示踪,因此研究超细颗粒物在生物体内的迁移过程对于研究大气颗粒物毒理具有重要意义.在本文中,采用近红外长余辉发光成像技术来研究超细颗粒物经肺暴露后在生物体体内的迁移示踪,选择了具备良好生物相容性的多孔二氧化硅为载体,负载具良好近红外长余辉发光性能的ZnGa_(2)O_(4)∶Cr(ZGO)模拟大气中的超细颗粒物(ZGO@SiO_(2)).研究结果表明,颗粒经由气管入肺后,会经循环系统迁移到体内其他器官,主要分布于心脏、肝脏和脾脏中,少量分布于肾脏,这可以为下一步研究超细颗粒物的生物毒理提供支持.

The ultra-fine particulate matters(less than 100 nm)are easy to enter into circulatory system of organism due to their small particle sizes,which would bring serious harm to organism.On the other hand,these ultra-fine particulate matters are difficult to be observed due to their small particle sizes.Thus,it is very important to investigate the migration behavior of ultra-fine particulate matters in organism.In this paper,we adopted the near-infrared long-term persistent luminescence imaging technology to visualize the migration behavior of ultra-fine particulate matters in organisms via the exposure of lung.Here,the mesoporous silica with good biocompatibility were chosen as templates to load near-infrared long-term persistent luminescence nanoparticles ZnGa_(2)O_(4)∶Cr to form ZGO@SiO_(2)nanospheres,which could be regarded as stimulated ultra-fine particulate matters.The obtained results showed that nanoparticles could easily enter into other organs after passing through the trachea into the lungs.The nanoparticles mainly distribute in the heart,liver,and spleen,while little distributed in the kidney.This long time in vivo imaging technique could provide much support for the further investigation on toxicity of ultra-fine particulate matters in the future.