蛋白核小球藻吸收无机砷的影响特征分析

Analysis on chemical characterization for inorganic arsenic uptake of Chlorella pyrenoidosa

蛋白核小球藻(Chlorella pyrenoidosa)由于其快速生长特性而被应用于水污染生态修复.本文以蛋白核小球藻为受试绿藻,研究了绿藻细胞界面对AsⅢ和AsⅤ的吸收累积作用,分析了水体pH影响,以及胞外聚合物(extracellular polymeric substance,EPS)释放量和官能团特征.结果表明,蛋白核小球藻细胞吸收AsⅢ和AsⅤ呈浓度依赖关系,短期暴露的藻生长抑制浓度AsⅢ低于AsⅤ,对AsⅢ和AsⅤ最大吸收量分别为39.84μg·g^-1和56μg·g^-1,其吸收和累积能力受pH影响,藻细胞藻-水界面zeta电位、EPS总释放量及其表面活性官能团被检测出呈特征变化.

Chlorella pyrenoidosa has been developed in bioremediation for aquatic ecosystem with more rapidly growth.In this study,C.pyrenoidosa was chosen and tested for the uptake process of AsⅢand AsⅤand the influences of pH levels,extracellular polymeric substance(EPS)production and EPS functional groups at the experiments of short-term arsenic exposure.The results showed that arsenic uptake capacities increased in a concentration-dependent manner,and the minimal exposure concentration of AsⅢfor inhibiting the growth of C.pyrenoidosa was lower than that of AsⅤ,the maximum uptake capacities of AsⅢand AsⅤwas 39μg·g^-1 and 56μg·g^-1,and were influenced by pH.In addition,physicochemical characteristics including the algal-water interface negative charge,EPS production and its surface functional groups were detected to be changed with the effects of arsenic exposure in Chlorella pyrenoidosa.