Fenton预氧化促进土壤微生物均衡降解烷烃

ACCELERATION OF VARIOUS ALKANES BALANCED DEGRADATION BY SOIL MICROORGANISMS WITH FENTON PRE-OXIDATION

针对石油污染土壤中各类烷烃的生物选择性降解问题,通过多种土壤固相铁Fenton预氧化方式调控土壤微生物群落,探究土壤微生物数量、活性和群落变化对石油烃降解的影响,确定各类烷烃均衡降解的微生物群落特征。结果表明:A45(45 mmol/L柠檬酸)和F8.7(8.7 mmol/L Fe^(2+))土壤固相铁Fenton预氧化后,土壤微生物代谢活性分别高达0.59 mol/kg(A45)和0.60 mol/kg(F8.7),土壤石油烃残余率分别低至30%(A45)和29%(F8.7)。土壤中形成以不动杆菌属(Acinetobacter)、假单胞菌属(Pseudomonas)为主要优势菌属的土壤微生物群落。土壤微生物多样性高,群落组成丰富,烷烃代谢的功能基因相对丰度高,促进了各类烷烃的均衡降解,各类烷烃的生物降解率均高达60%。

Aiming at selective biodegradation of various alkanes in the petroleum contaminated soil,microbial communities in the soil were screened by regulating multiple soil solid phase Fe Fenton pre-oxidation.Then,the influence of soil microbial quantity,activity and community changes on the degradation of petroleum hydrocarbons was explored,and the microbial community characteristics for the balanced degradation of various alkanes were determined.The results showed that:after Fenton pre-oxidation of solid phase Fe with A45(45 mmol/L citric acid)and F8.7(8.7 mmol/L Fe^(2+)),the metabolic activity of soil microorganisms was as high as 0.59 mol/kg(A45)and 0.60 mol/kg(F8.7),respectively.The petroleum hydrocarbon residual rate in the soil was reduced to 30%(A45)and 29%(F8.7),respectively.At the same time,there were soil microbial communities containing Acinetobacter,and Pseudomonas as dominant bacterial strains in the soil.The diversity of soil microorganisms was high,the community composition was rich,and the abundance of functional genes in alkane metabolism was high.Based on this situation,the balanced degradation of various alkanes was realized and promoted,and the biodegradation of various alkanes in the soil all reached 60%.