NaYF_(4)∶Yb,Er纳米粒子的合成及免疫传感器的制备与应用

Synthesis of NaYF_(4):Yb,Er nanoparticles and the preparation and application of immunosensor

稀土掺杂发光纳米材料具有独特的性质,如发射寿命长、发射波段窄、Stokes位移大、光稳定性强、荧光寿命长、反自发荧光能力强等特点,为进一步探索稀土纳米粒子在生物传感器方面的应用,首先研究上转换稀土纳米粒子的可控合成,后采用巯基乙酸(TGA)对其表面进行改性,再将其固癌胚抗原制备免疫传感器,进行了电化学发光测试和样品检测。结果表明:溶剂法合成的纳米粒子形貌均一,分散性好,构建的免疫传感器的检测极限低达3.2pg/mL。

Luminescent nanomaterials doped with rare earth have the characteristics of long emission lifetime,narrow spontaneous light spectrum,large stokes shift,good light stability,long fluorescence lifetime and strong anti-spontaneous fluorescence ability etc.In order to further explore the application of rare earth nanoparticles in biosensors,the controllable synthesis of upconversion rare earth nanoparticles was firstly investigated,and then the surface was modified by thioglycolic acid(TGA).Finally,an immunosensor was prepared using the solid phase carcinoembryonic antigen,and the sample characterization and electrochemiluminescence were carried out.The results showed that the nanoparticles synthesized by the solvent method had uniform morphology and good dispersibility,and the constructed immunosensor had the detection limit as low as 3.2pg/mL.