放线菌HBERC-39158抗真菌活性化合物的分离鉴定

Isolation and identification of antifungal active compounds of actinomycete HBERC-39158

以从四川省乐山市采集土样中分离的1株放线菌HBERC-39158为研究对象,对该菌株进行了16S分子鉴定、摇瓶发酵及室内生物活性测定,并对发酵提取物进行HPLC制备,获得了不同时间流出的样品36份,经组分活性测定,确定了其活性部位,并制备了活性化合物纯品,对其进行了HPLC-MS及NMR分析。结果表明,该菌株为链霉菌(Streptomyces kronopolitis),提取物对番茄灰霉病菌(Botrytis cinerea)、小麦颖枯病菌(Septoria nodorum)、番茄早疫病菌(Alternaria solani)、立枯丝核菌(Rhizoctonia solani)等重要农作物病原真菌具有良好活性,其活性组分出现在14~19 min。根据活性化合物紫外吸收光谱、质谱数据以及核磁共振谱的解析,结合文献比对,确定其活性化合物为factumycin。

The antifungal activity and active compound of an actinomycete strain HBERC-39158 were studied,which was isolated from soil samples collected from Leshan city,Sichuan province. After 16 S sequencing,shaking flask fermentation and bioactivity determination,the fermentation extract was prepared by HPLC,and it was divided into 36 components based on outflow times. By component activity test,the active site was clarified,and the pure active compound was isolated by preparative HPLC. The compound was analyzed by HPLC-MS and NMR. The results showed that the strain HBERC-39158 was identified as Streptomyces kronopolitis,the fermentation extract showed good activity on pathogenic fungi of crops,such as Botrytis cinerea,Septoria nodorum,Alternaria solani,Rhizoctonia solani,and its active components appearred in 14~19 min. Based on the analysis of UV absorption spectra,MS data and NMR spectra of active compound,combined with literature comparison,the active compound was identified as factumycin.