磷源对聚磷污泥和反硝化聚磷污泥微生态影响

Effects of Phosphorus Sources on Micro-ecology of Activated Sludge Cultivation by Oxygen and Nitrate

活性污泥微生物活动是去除污染的核心驱动力。该文以7种不同的磷化合物作为单一磷源,对常规活性污泥(O-S活性污泥)和以硝酸盐作为电子受体驯化后的活性污泥(N-S活性污泥)的微生态影响差异进行了研究。结果显示,对于2种活性污泥系统,磷酸钠、三偏磷酸钠和三聚磷酸钠的优势菌均为Pseudomonas,经过60 h的培养,其占比分别达到62.33%、61.07%和52.23%(O-S系统)、68.38%和63.41%(N-S系统)。磷酸肌酸优势菌由Candidatus Accumulibacter转变为Pseudomonas,功能基因pho A和pho U丰度大幅度提高,分别达O-S系统的3.52倍和1.54倍。D-葡萄糖-6-磷酸和2-磷酸甘油酸在以硝酸盐为电子受体活性污泥作用下,其优势菌转变为Rhizobium(7.38%和17.20%)。这些结果表明,活性污泥经不同电子受体驯化后,其微生态不同,与碱性磷酸酶相关的功能基因丰度也发生了变化。

Microorganisms in activated sludge are essential for pollutants removal.In this paper,the difference in micro-ecology of activated sludge cultivation by oxygen(O-S)and nitrate(N-S)with seven types phosphorus was studied.The dominant bacteria of sodium phosphate,sodium trimetaphosphate and sodium tripolyphosphate reached Pseudomonas,culturing for 60 hours,the proportion of which was 62.33%,61.07%and 52.23%(O-S system),68.38%and63.41%(N-S system),respectively.The dominant bacteria of creatine phosphate acid changed from Candidatus accumulibacter to Pseudomonas,and the abundance of AKP-associated genes—pho A and pho U increased significantly,which was 3.52 times and 1.54 times of that of O-S system,respectively.With the action of activated sludge of nitrate as electron receptor,the dominant bacteria of D-glucose-6-phosphate and 2-phosphoglyceric acid were transformed into Rhizobium(7.38%and 17.20%).The results illustrated that the micro-ecology of activated sludge was different after cultivation with different electron receptors,and the abundance of functional genes related to alkaline phosphatase also changed.