基于SRAP和SCoT标记分析不同甜樱桃品种遗传多样性

SRAP and SCoT Markers-based Analysis on Genetic Diversity of Sweet Cherry Cultivars

【目的】探究40个甜樱桃(Prunus avium L.)品种的遗传多样性及SRAP和SCoT标记在甜樱桃上的应用。【方法】利用SRAP和SCoT分子标记进行遗传多样性分析。【结果】筛选出6对条带清晰、多态性好的SRAP引物和7条SCoT引物,在40个甜樱桃品种中分别扩增出多态性条带67和69条,多态性百分率分别为90.54%和93.24%。SRAP标记和SCoT标记的UPGMA聚类分析表明,40个甜樱桃品种的遗传相似系数分别在0.67~0.95和0.72~0.93,说明甜樱桃的遗传背景相对较窄。SRAP标记在相似系数0.79左右可以将40份甜樱桃分为6组,SCoT标记在相似系数在0.77左右可以将40份甜樱桃分为6组。两种分子标记中,来自不同地区的甜樱桃品种没有明显聚类,说明各个地区甜樱桃品种基因交流频繁,另外大部分黄色系甜樱桃品种聚为一类。【结论】2种分子标记均可应用于分析甜樱桃遗传多样性且能够区分不同果皮颜色甜樱桃,可以作为后期种质资源利用和新品种选育的技术手段。

【Objective】Genetic relationship of 40 sweet cherry cultivars were analyzed.【Methods】SRAP and SCoT molecular markers were used to determine the genetic diversity of sweet cherry varieties.【Results】Six pairs of SRAP primers and 7 pairs of SCoT primers with distinct bands and polymorphism were selected as the markers to obtain 67 and 69 amplified bands representing 90.54%and 93.24%of polymorphism,respectively,from the 40 cultivars.Based either on SRAP with a similarity coefficient of about 0.79 or on SCoT with a similarity coefficient of about 0.77,the cultivars could be divided into 6 groups.Thus,the sweet cherry cultivars collected from different regions could have gone through numerous genetic exchanges becoming low in variation.Therefore,not surprisingly,most of the yellow varieties were grouped into one single category.【Conclusion】The SRAP and SCoT markers successfully helped examine the genetic diversity of 40 sweet cherry cultivars collected from various regions.The result obtained would facilitate further studies in the germplasm utilization and breeding of sweet cherries.