嘉宝果叶片对高血糖小鼠的降血糖作用

Hypoglycemic Effect of Jaboticaba Leaf on Hyperglycemic Mice

【目的】研究嘉宝果叶片的体内降糖功效,为嘉宝果降糖功能因子开发提供理论依据。【方法】采用尾静脉注射链脲佐菌素(STZ)制备高血糖小鼠模型。将模型小鼠随机分为模型对照组和嘉宝果叶片处理组(叶片添加比例1%、2%、4%和8%),并设正常小鼠对照组,持续饲喂30 d,测定不同时间段各处理小鼠的空腹血糖值(FBG),分析小鼠的血糖变化情况,测定各组小鼠的糖耐量、甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白-胆固醇(LDL-C)、高密度脂蛋白-胆固醇(HDL-C)的含量。【结果】嘉宝果叶片具有显著的降血糖效果,随着嘉宝果叶片添加剂量增加,降血糖效果逐渐增强。与模型对照组比较,嘉宝果叶片8%添加组的小鼠FBG极显著降低(P<0.01),4%和8%添加组的小鼠糖耐量极显著改善(P<0.01)。各叶片添加组均能升高HDL-C水平(P<0.01),降低TG水平(P<0.05)。【结论】在饲料中添加4%和8%的嘉宝果叶片对STZ诱导的高血糖小鼠具有良好的降血糖作用,并能改善脂质代谢。

【Objective】Hypoglycemic effect of jaboticaba leaf was studied on diabetic mice artificially induced by streptozotocin(STZ)injection.【Method】Mice were intravenously injected with STZ and randomly divided into hyperglycemic control and 4 treatment groups.Mice in the treatment groups were each fed with a diet that included 1%,2%,4%or 8%of jaboticaba leaves.In addition,another control group of healthy mice was included for comparison.During the 30 d test at intervals after administration,fasting blood glucose(FBG)of each group was measured to monitor the changes over time.Glucose tolerance and serum lipid indicators including triglyceride(TG),total cholesterol(TC),low density lipoproteincholesterol(LDL-C),and high density lipoprotein-cholesterol(HDL-C)of each animal were determined at end of the feeding test.【Result】Jaboticaba leaves exhibited significant hypoglycemic effect on the hyperglycemic mice.The effect increased with increasing dosage of jaboticaba leaves.Compared with the mice in the hyperglycemic control group,the treatment mice fed with 8%jaboticaba leaves significantly decreased on FBG(P<0.01);those fed with 4%and 8%jaboticaba leaves significantly improved on glucose tolerance(P<0.01);those in all treatment groups elevated on HDL-C(P<0.01)and decreased on serum TG(P<0.05).【Conclusion】When jaboticaba leaves were added to the feed at 4%or 8%level,significant hypoglycemic effect as well as improved lipid metabolism were observed on the STZ-induced hyperglycemic mice.