超声波辅助酶法提取油茶籽油的影响因素研究

Study on impact factors of the ultrasound-assistedenzymatic aqueous extraction of oil from tea seed

主要研究了超声波技术辅助水酶法提取油茶籽油的工艺。比较不同粉碎条件、不同超声条件及多种酶的油茶籽油提取率,确定最佳工艺:物料粉碎粒度为0.600 mm,超声功率400 W,超声温度40℃,超声时间40 min;最适酶依次为:Alcalase 2.4L>Cellulase>Protex 7L>Viscozyme L。在最佳工艺条件下,游离油提取率达到59.23%,总油提取率达到94.56%。

The present study aimed to evaluate the process of ultrasound-assisted enzymatic aqueous extraction of oil from tea seeds.The optimization procedure was performed on different crushing conditions,ultrasound conditions,and enzymes.The optimized parameters were as follows:crushing particle size 0.600 mm,ultrasonic power 400 W,ultrasonic temperature 40℃,ultrasonic time 40 min.The order of enzyme efficiency was Alcalase 2.4L>Cellulase>Protex 7L>Viscozyme L.Under the above optimum conditions,the free oil extraction rate was 59.23%,and the total oil extraction rate was 94.56%.