桑螟触角中谷胱甘肽硫转移酶基因的鉴定及组织表达谱分析

Identification of Glutathione S-Transferase Genes in Glyphodes pyloalis Antennae and Analysis on Their Tissue Expression Profile

桑螟(Glyphodes pyloalis)是桑树的主要害虫,严重危害各蚕桑主产区的桑叶产量。从桑螟触角转录组数据库中共鉴定出15个谷胱甘肽硫转移酶(GST)基因。序列分析显示,这些基因均具有完整的开放阅读框,与其他鳞翅目昆虫的GST基因具有高度的相似性,其中8个GST基因具有谷胱甘肽结合域。系统进化树显示这些GST基因分别归属于GST的6个亚类。荧光定量PCR结果表明,GpGST-D1在雌、雄成虫触角中高度特异性表达,推测可能参与桑螟触角中气味降解过程。GpGST-O1和GpGST-O2在雌成虫触角中的表达量高于其他组织,GpGST-D3在雌成虫腹部的表达量最高,但在触角中的表达量与雄成虫无显著差异。除GpGST-D3外,10个GpGST(GpGST-D2、GpGST-D4、GpGST-E1、GpGST-E2、GpGST-E3、GpGST-T1、GpGST-Z1、GpGST-S1、GpGST-S2、GpGST-S3)在桑螟成虫腹部的表达量也高于其他组织,但呈现出性别差异。研究结果为深入了解桑螟嗅觉感受行为的分子机制提供了基础,也为研发新型桑螟防治手段提供了参考。

Glyphodes pyloalis as a major pest of mulberry can cause serious damages to mulberry production in main sericultural areas.In this study,a total of 15 glutathione S-transferase(GST)genes were identified from transcriptome data of antennae in G.pyloalis,all of which have complete open reading frames and are highly similar to GST genes of other Lepidoptera insects according to sequence analysis.Eight GST genes have glutathione binding domain.Phylogenetic analysis revealed that these GST genes belong to 6 GST sub-classes.Real-time qPCR results indicated that GpGST-D1 was highly expressed in antennae of female and male adults,which suggested that it might be involved in degradation process of odor in G.pyloalis antennae.Beside,expression levels of GpGST-O1 and GpGST-O2 were higher in antennae than in other tissues of female adult,and expression level of GpGST-D3 was the highest in abdomen of female adult.However,there was no significant difference in expression level of GpGST-D3 in antenna between female and male adult.Apart from GpGST-D3,10 GpGSTs(GpGST-D2,Gp-GSTD4,GpGST-E1,GpGST-E2,GpGST-E3,GpGST-T1,GpGST-Z1,GpGST-S1,GpGST-S2,GpGST-S3)were all higher expressed in abdomen than in other tissues in adult G.pyloalis though they showed sex-biased differences.Results of this study deepened our understanding on molecular mechanism of olfactory behavior of G.pyloalis,and help to develop new control way against G.pyloalis.