稀土Tb^(3+)为荧光探针定量分析色氨酸

Rare earth Tb^(3+) as a fluorescent probe for the quantitative analysis of tryptophan

提出Tb^(3+)荧光可用于色氨酸检测.利用Tb^(3+)和色氨酸之间的能量转移,建立Tb^(3+)—色氨酸体系,在547 nm处对该体系进行分析,并将其应用于花生中色氨酸的检测.结果发现:色氨酸在0.1~16 mmol·L^(-1)浓度范围内与Tb^(3+)的荧光强度呈线性关系,回归方程为:I=1.096 C+20.37.因此,稀土Tb^(3+)可以对色氨酸进行定量分析.此外,测定了花生中的色氨酸含量,并进行了加标回收实验,回收率为96.90%~103.42%.

Rare earth terbium(Tb^(3+))has strong fluorescence effect and therefore can be used as a fluorescent probe.With the wide application of rare earth fluorescent probes,it is of practical significance to apply them to the detection of amino acids.Herein,tryptophan was detected by direct Tb^(3+)fluorescent probe method.Tb^(3+)-tryptophan system was established using the energy transfer between Tb^(3+)and tryptophan,which was analyzed at 547 nm.This was applied to the detection of tryptophan in peanuts.The results showed that there was a linear relationship between tryptophan and the fluorescence intensity of Tb^(3+)in the concentration range of 0.1 mmol·L^(-1)~16 mmol·L^(-1),and the regression equation was:I=1.096 C+20.37.Therefore,rare earth Tb^(3+)could quantitatively analyze tryptophan.In addition,the tryptophan content in peanuts was determined.A standard addition recovery test was carried out,and the recovery rate was 96.90%~103.42%.