猪苓提取物对小鼠肉瘤180瘤细胞的^(3)H胸腺嘧啶核苷掺入和cAMP磷酸二酯酶的影响

THE EFFECT OF THE EXTRACT OF CHU-LING(POLYPORUS UMBELLATA)ON THE INCORPORATION OF ^(3)H THYMIDINE AND THE ACTIVITY OF cAMP PHOSPHODIESTERASE OF SARCOMA 180 ASCITES TUMOR CELLS.

本实验研究了猪苓提取物对小鼠肉瘤180腹水瘤细胞的影响。实验动物每天向腹腔注射100~200毫克/公斤体重剂量的猪苓提取物。从细胞化学及放射自显影等的研究结果表明:(一)猪苓提取物对于腹水瘤细胞内的3′,5′cAMP磷酸二酯酶活性具有显著抑制效果,腹水瘤细胞的标记指数(进行DNA合成的细胞数)明显降低。(二)观察到猪苓提取物对于腹水瘤细胞的增殖以及3′,5′cAMP磷酸二酯酶的活性具有平行的抑制效果。根据上述实验结果,本文还讨论了猪苓提取物与腹水瘤细胞的3′,5′cAMP磷酸二酯酶的活性以及cAMP水平之间的关系。

The effect of the extract of Chu-Ling on sarcoma 180 ascites tumor cells in mice was investigated.Experimental mice were injected intraperitoneally at a dose of 100-200 mg/kg body weight/day with the extract of Chu-Ling.Cytochemical and autoradiographic studies etc.revealed the following:First,there were obviously inhibitory effect of the extract of Chu-Ling on the activity of 3′,5′cAMP-PDE in ascites tumor cells,and a marked decrease in the labelled index(The percentage of cells synthesizing DNA)of ascites tumor cells.Secondary,parallel inhibitory effects of the activity of 3′,5′cAMP-PDE and proliferation of ascites tumor cells were observed.Also,the effect of the extract of Chu-Ling on the relationship between the activity of 3′,5′cAMP-PDE and cAMP level in ascites tumor cells isdiscussed.