黑木耳多糖提取液浊度清除的响应面优化

Optimization of Turbidity Removal Technology for Extracts From Auricularia auricula Polysaccharide by Response Surface Methodology

以黑木耳粗多糖提取液为试材,采用比浊法和苯酚-硫酸法,研究了提取液的酸度、活性白土用量、处理温度和时间对浊度清除率和多糖回收率的影响,利用Box-Behnken试验设计建立并分析了各因素对浊度清除率和多糖回收率影响的数学模型,以期为黑木耳多糖提取液的合理开发与利用提供参考依据。结果表明:经活性白土处理后,黑木耳多糖提取液中的蛋白质含量由0.39 mg·mL^(-1)降为0.05 mg·mL^(-1)。活性白土处理多糖提取液浊度的最优工艺条件为在pH 7.0的条件下,按料液比1.86 g·mL^(-1)添加活性白土,于44.8℃处理61.2 min,多糖回收率和浊度清除率分别为85.7%和61.0%。

The effects of acidity of the extract,the amount of activated clay,the treatment temperature and time on the clearance efficiency of turbidity and the recovery of polysaccharide were investigated by using the extract of polysaccharides fromAuricularia auriculariaand evaluation with turbidimetry and phenol-sulfuric acid methods.Box-Behnken experiment design was employed to establish and analyze the mathematical model of the influence of each factor on the removal efficiency of turbidity and the recovery of crude polysaccharide,in order to provide reference for the rational development and utilization of the extract of crude polysaccharides from Auricularia auricularia.The results showed that the protein content in the extract was reduced from 0.39 mg·mL^(-1)to 0.05 mg·mL^(-1)after treatment with activated clay.The optimal process conditions for removal of the turbidity in the extract were as follows,the recovery of polysaccharides and removal efficiency of turbidity were85.7%and 61.0%respectively by addition of activated clay at a solid-liquid ratio of 1.86g·mL^(-1)to the extract(pH 7.0)and treatment at 44.8℃for 61.2 minutes.