一株野生黄伞的菌种分离、鉴定及生物学特性

Isolation, Identification and Biological Characteristics of a Wild Pholiota adiposa

以采集于柳树上的DL711菌株为研究对象,依据形态学特征、出菇试验及ITS序列分析对该菌种进行鉴定,同时采用单因素试验方法,研究了不同营养成分及培养条件对菌丝生长的影响,以期为食用菌开发利用提供菌种资源。结果表明:该菌株经鉴定为黄伞(Pholiota adiposa),其菌丝生长的最适宜碳源为果糖,最适氮源为酵母膏,最适宜无机盐为磷酸二氢钾,最适生长温度为25℃,最适宜pH为4~7,维生素对菌丝生长影响不大。

Taking DL711 strain collected from willow as the research object,the strain was identified according to morphological characteristics,fruit-formed test and ITS sequence analysis.Meanwhile,single factor test was used to study the effects of different nutrient components and culture conditions on mycelial growth,in order to provide strain resources for the development and utilization of edible fungi.The results showed that the strain was identified as Pholiota adiposa.The most suitable carbon source for mycelial growth was fructose,the most suitable nitrogen source was yeast extract,the most suitable inorganic salt was potassium dihydrogen phosphate,the most suitable growth temperature was 25℃,the most suitable pH range was 4-7,and vitamins had little effect on mycelial growth.