海藻糖浸种对不同盐胁迫下甜瓜种子萌发的影响

Effects of Seed Soaking With Exogenous Trehalose on the Germination of Melon Under Different Saline Conditions

以‘西州蜜25号’甜瓜为试材,采用不同浓度(2.5、5.0、10.0、20.0mmol·L^(-1))外源海藻糖浸种处理,研究海藻糖预处理甜瓜种子对不同浓度NaCl和混合盐溶液条件下种子萌发及幼苗生长的影响,以期为甜瓜栽培生长提供参考依据。结果表明:NaCl和混合盐溶液浓度的增高,甜瓜种子发芽能力受到抑制,超过75mmol·L^(-1)浓度盐胁迫的甜瓜种子萌发过程中的发芽率和发芽势等指标显著下降;而海藻糖浸种则可以缓解75mmol·L^(-1)盐处理对甜瓜种子的伤害,其萌发相关指标均得到了提升;在150mmol·L^(-1)盐胁迫下,不同浓度海藻糖预处理对甜瓜种子萌发缓解作用不明显;通过隶属函数分析比较发现,浓度为2.5~5.0mmol·L^(-1)海藻糖对甜瓜种子萌发与幼苗地上及地下部长度的提高均显著,对盐胁迫具有缓解效应,故效果最佳。

Taking‘Xizhoumi No.25’melon as test materials,the effect of exogenous trehalose on melon seed germination,seedling growth under different NaCl and complex saline condition was studied by using different concentrations(2.5,5.0,10.0,20.0mmol·L^(-1))solution of trehalose,in order to provide reference for the melon production.The results showed that germination index and length of the seedlings gradually decreased with increasing concentration of the different salinity.The germination rate and germination potential of melon seeds were significantly decreased when the salinity was>75mmol·L^(-1);and trehalose soaked seeds could alleviate the damage of 75mmol·L^(-1)saline treatment on melon seeds,and the germination related indexes were improved.Pretreatment with different concentrations trehalose had no significant effect on melon seed germination under 150mmol·L^(-1)treatment of different salinity.Through the analysis and comparison of membership functions,soaking within a trehalose concentration range from 2.5mmol·L^(-1)to 5.0 mmol·L^(-1)significantly increased seed germination parameters under 75mmol·L^(-1)treatment of salinity.In a word,soaking with low concentration trehalose could effectively alleviate the inhibitory effects of salinity stress on seed germination.