黑果腺肋花楸花色苷的提取分离纯化工艺

Extraction and Purification Techniques of Anthocyanins From Aronia melanocarpa

以黑果腺肋花楸为试材,采用Box-Behnken响应面法优化及柱层析法分离纯化花色苷,以确定花色苷的最佳提取工艺。结果表明:花色苷最佳提取工艺为浸提温度46℃、料液比1∶23.8g·mL^-1、浸提时间4.2h,其提取率为6.12mg·g^-1;最佳分离纯化工艺为以XAD-7型树脂为填料,30%乙醇洗脱,最佳吸附流速1.0 mL·min^-1,解析流速1.0mL·min^-1。在此基础上,可对黑果腺肋花楸花色苷进行微胶囊化,以便应用到食品加工中。

Aronia melanocarpa was used as material,Box-Behnken response surface methodology and column chromatography were used to extract and purify anthocyanin,in order to obtain the optimal extraction process.The results showed that the optimal extraction process of anthocyanins was the extraction temperature 46℃,material-liquid ratio 1∶23.8 g·mL^-1,extraction time 4.2 hours,the extraction rate was 6.12 mg·g^-1;the best separation and purification process was XAD-7 resin as filler,eluted with 30%ethanol,the optimal adsorption flow rate was 1.0 mL·min^-1,and the analytical flow rate was 1.0 mL·min^-1.On this basis,the anthocyanins of antarctica costatum could be microencapsulated for application in food processing.