利用正交实验优选蓝莓“比洛克西”组织培养条件

Optimum Tissue Culture Conditions of Blueberry ’Biloxi’ by Orthogonal Test

以南方高丛蓝莓"比洛克西"半木质化茎段为外植体,利用正交实验方法,高效优选其组织培养最佳条件。结果表明:外植体的最佳处理方法为先用75%乙醇消毒45s,再用10%NaClO消毒10min,然后把茎段接种到含WPM+ZT 1.00mg·L^-1的初代诱导培养基中;增殖培养的最佳培养基为WPM+6-BA 1.00mg·L^-1+ZT 2.00mg·L^-1+IBA 0.25 mg·L^-1,壮苗培养的最佳培养基为WPM+ZT 0.50 mg·L^-1+IBA0.50mg·L^-1;生根诱导的最佳培养基质为WPM+蛭石∶椰糠(V 1∶1)+活性炭3.00g·L^-1+IBA 0.500mg·L^-1。

The semi-hardwood stem segments of southern high-bush blueberry’Biloxi’were used as explants,and the optimum conditions for tissue culture were optimized by orthogonal test.The results showed that the best disinfection method for explants was to disinfect them with 75%ethanol for 45 seconds,then 10%NaClO for 10 minutes,and then put stems into WPM+ZT 1.00 mg·L^-1 medium;the optimum medium for propagation culture was WPM+6-BA 1.00 mg·L^-1+ZT2.00 mg·L^-1+IBA 0.25 mg·L^-1;the optimum medium for strong seedling culture was WPM+ZT0.50 mg·L^-1+IBA 0.50 mg·L^-1;and the optimum medium for rooting was WPM+vermiculite∶coconut coir(volume ratio 1∶1)+activated carbon 3.00 g·L^-1+IBA 0.500 mg·L^-1.