摘要
Objective:To investigate the antioxidant and anti-melanogenesis activities of an ultrasonic extract of red sea cucumber,Stichopus japonicus,collected from Jeju Island.Methods:Antioxidant activity experiments were assessed by an electron spin resonance system and a cellular model of immortalized human keratinocytes(HaCaT)to determine its radical scavenging activity and protective effects against 2,2’-azobis(2-amidinopropane)dihydrochloride(AAPH)-induced oxidative stress.Antimelanogenic activity of the ultrasonic extract of red sea cucumber was also examined using the melanoma cell model B16F10 and mushroom tyrosinase.Following the induction byα-melanocytestimulating hormone,the effects of the ultrasonic extract of red sea cucumber on intracellular tyrosinase activity,melanin content and the melanogenic protein expression of microphthalmia-associated transcription factor,tyrosinase,and tyrosinase-related proteins(TRP-1,and TRP-2)were examined.Results:The ultrasonic extract of red sea cucumber significantly scavenged 2,2-diphenyl-1-picrylhydrazyl and alkyl radicals[IC50:(0.924±0.035)and(0.327±0.006)mg/mL,respectively],as well as showed a protective effect against oxidative stress and attenuated generation of intracellular reactive oxygen species on AAPHinduced HaCaT cells,with no cytotoxicity(12.5-400μg/mL).The ultrasonic extract of red sea cucumber also exhibited a tyrosinase inhibitory effect[IC50:(2.750±0.006)mg/mL].Onα-melanocytestimulating hormone-stimulated B16F10 melanoma cells,the ultrasonic extract of red sea cucumber(25-200μg/mL)significantly inhibited not only melanin synthesis and tyrosinase activity,but also protein expressions of microphthalmia-associated transcriptional factor,tyrosinase,TRP-1,and TRP-2.Conclusions:The ultrasonic extract of red sea cucumber shows antioxidant and anti-melanogenic potential and may be a natural candidate for anti-aging as well as a whitening agent in the cosmeceuticals industry.
Objective: To investigate the antioxidant and anti-melanogenesis activities of an ultrasonic extract of red sea cucumber, Stichopus japonicus, collected from Jeju Island.Methods: Antioxidant activity experiments were assessed by an electron spin resonance system and a cellular model of immortalized human keratinocytes（HaCaT） to determine its radical scavenging activity and protective effects against 2,2’-azobis（2-amidinopropane） dihydrochloride（AAPH）-induced oxidative stress. Antimelanogenic activity of the ultrasonic extract of red sea cucumber was also examined using the melanoma cell model B16F10 and mushroom tyrosinase. Following the induction by α-melanocytestimulating hormone, the effects of the ultrasonic extract of red sea cucumber on intracellular tyrosinase activity, melanin content and the melanogenic protein expression of microphthalmia-associated transcription factor, tyrosinase, and tyrosinase-related proteins（TRP-1, and TRP-2） were examined. Results: The ultrasonic extract of red sea cucumber significantly scavenged 2,2-diphenyl-1-picrylhydrazyl and alkyl radicals [IC50:（0.924±0.035） and（0.327±0.006） mg/mL, respectively], as well as showed a protective effect against oxidative stress and attenuated generation of intracellular reactive oxygen species on AAPHinduced HaCaT cells, with no cytotoxicity（12.5-400 μg/mL）. The ultrasonic extract of red sea cucumber also exhibited a tyrosinase inhibitory effect [IC50:（2.750±0.006） mg/mL]. On α-melanocytestimulating hormone-stimulated B16F10 melanoma cells, the ultrasonic extract of red sea cucumber（25-200 μg/mL） significantly inhibited not only melanin synthesis and tyrosinase activity, but also protein expressions of microphthalmia-associated transcriptional factor, tyrosinase, TRP-1, and TRP-2. Conclusions: The ultrasonic extract of red sea cucumber shows antioxidant and anti-melanogenic potential and may be a natural candidate for anti-aging as well as a whitening agent in the cosmeceuticals industry.
基金
supported by a research grant funded by the Korea Institute of Ocean Science and Technology(PE99722)
supported by Soonchunhyang University Research Fund.
