摘要
Objective:To evaluate spasmolytic mechanisms of aqueous and methanolic extracts from Distemonanthus benthamianus trunk-bark.Methods:Spasmolytic activities of extracts were evaluated in vitro on spontaneous and potassium chloride-induced jejunum contractions,or against cholinergic[acetylcholine(0.3μmol/L)]stimulations.High performance liquid chromatography analysis of both extracts was performed in reference to standard compounds.Results:Extracts developed concentration-dependent inhibitory activities.The methanolic extract,which revealed better activity,produced spasmolytic and myorelaxant effects at concentrations of 0.01-0.30 mg/mL with EC(50)of 0.06 and 0.09 mg/mL(95%CI:0.03-0.3 mg/mL),respectively.Its anticholinergic effect was obtained at the same concentrations with EC(50)of 0.11 mg/mL(95%CI:0.03-0.3 mg/mL).Chromatograms showed the presence of gallic acid in both extracts,rutin being only detected in the aqueous extract.Conclusions:Distemonanthus benthamianus extracts exhibit verapamil and atropine-like activities,thus highlighting calcium channels and muscarinic receptors blocking potentials,which may be conveyed by some phenolic compounds.These results confirm the antidiarrheal activity of Distemonanthus benthamianus extracts.
Objective: To evaluate spasmolytic mechanisms of aqueous and methanolic extracts from Distemonanthus benthamianus trunk-bark. Methods: Spasmolytic activities of extracts were evaluated in vitro on spontaneous and potassium chloride-induced jejunum contractions, or against cholinergic [acetylcholine(0.3 μmol/L)] stimulations. High performance liquid chromatography analysis of both extracts was performed in reference to standard compounds. Results: Extracts developed concentration-dependent inhibitory activities. The methanolic extract, which revealed better activity, produced spasmolytic and myorelaxant effects at concentrations of 0.01-0.30 mg/mL with EC(50) of 0.06 and 0.09 mg/mL(95% CI: 0.03-0.3 mg/mL), respectively. Its anticholinergic effect was obtained at the same concentrations with EC(50) of 0.11 mg/mL(95% CI: 0.03-0.3 mg/mL). Chromatograms showed the presence of gallic acid in both extracts, rutin being only detected in the aqueous extract. Conclusions: Distemonanthus benthamianus extracts exhibit verapamil and atropine-like activities, thus highlighting calcium channels and muscarinic receptors blocking potentials, which may be conveyed by some phenolic compounds. These results confirm the antidiarrheal activity of Distemonanthus benthamianus extracts.
基金
supported by the CIIT-TWAS Sandwich Postgraduate Fellowship(FR number:3240293217,2016).
