摘要
[Objectives]To investigate the effect of low temperature treatment on cotton leaves at the two-leaf stage.[Methods]Two cotton varieties(CN01 and SJB016(low temperature-tolerant))were used as the trial materials.They were treated at 25(CK)and 12℃(low temperature)for 0,3,6,12,24,48 and 72 h,respectively.Then,the changes in the contents of MDA,SS and Pro in the cotton leaves were analyzed.Based on the analysis results,RNA-seq verification was performed.[Results]Two cotton varieties(CN01 and SJB016(low temperature-tolerant))were used as the trial materials.They were treated at 25(CK)and 12℃(low temperature)for 0,3,6,12,24,48 and 72 h,respectively.Then,the changes in the contents of MDA,SS and Pro in the cotton leaves were analyzed.Based on the analysis results,RNA-seq verification was performed.[Conclusions]These genes may play an important role in improving the cold resistance of cotton.
基金
Supported by Integration and Demonstration Project of Simple and Efficient Cultivation Technology of Cotton(2020YFD1001000)
Special Fund for Construction of Modern Agricultural Industrial Technology System
Project of Hubei Agricultural Science and Technology Innovation Center(2019-620-000-06)
