香菇多糖对基于维生素D-成纤维细胞生长因子23-Klotho蛋白轴中维生素D缺乏大鼠骨代谢的保护作用

Protective Effects of Lentinan on Bone Metabolism in Vitamin D Deficient Rats via Vitamin D-fibroblast Growth Factor-23-Klotho Axis

探究香菇多糖基于维生素D-FGF-23-Klotho轴对维生素D缺乏大鼠骨代谢的影响。选取50只SD健康大鼠,10只作为对照组,其余40只建立维生素D缺乏模型,分为模型组、维生素D3组(1000 U/kg)、低剂量香菇多糖组(150 mg/kg)和高剂量香菇多糖组(450 mg/kg)各10只。检测大鼠血清钙、磷、碱性磷酸酶水平、维生素D-FGF-23-Klotho轴因子水平、骨代谢指标水平。研究结果显示,对照组大鼠血清25(OH)VD3、1,25(OH)VD3、FGF-23、Klotho水平分别为(75.58 ng/mL、4.98 ng/mL、468.25 pg/L、62.58pg/mL),模型组大鼠25(OH)VD3、1,25(OH)VD3、Klotho下降至(34.35 ng/mL、3.52 ng/mL、40.12 pg/mL),FGF-23水平上升至(896.35pg/L),与对照组相比差异具有统计学意义(p<0.05)。高剂量香菇多糖组大鼠25(OH)VD3、1,25(OH)VD3、Klotho水平上升至(71.24 ng/mL、4.85 ng/mL、58.68 pg/mL),统计上高于维生素D3组、低剂量香菇多糖组,FGF-23下降至(552.23pg/L),统计上低于维生素D3组、低剂量香菇多糖组(p<0.05)。香菇多糖通过作用于维生素D-FGF-23-Klotho轴,调控轴因子,增加维生素D的含量,改善因维生素缺乏所导致的成骨障碍,起到保护维生素D缺乏大鼠骨质的作用。

To investigate the effect of Lentinan on bone metabolism in vitamin D deficient rats via vitamin D-fibroblast growth factor-23-Klotho axis,fifty SD healthy rats were selected,with 10 as control group,and the remaining 40 as the vitamin D deficient models(which were divided into the model group,vitamin D3 group(1000 U/kg),low-dose lentinan group(150 mg/kg)and high-dose lentinan group(450 mg/kg),with 10 rats per each group).The levels of serum calcium,phosphorus,alkaline phosphatase,vitamin D-fibroblast growth factor(FGF)-23-Klotho axis factors and bone metabolism indices were measured.The results showed that the serum levels of 25(OH)VD3,1,25(OH)VD3,FGF-23 and Klotho in the control group were 75.58 ng/mL,4.98 ng/mL,468.25 pg/L,62.58 pg/mL,respectively,while for the model group,the 25(OH)VD3,1,25(OH)VD3 and Klotho decreased to 34.35 ng/mL,3.52 ng/mL and 40.12 pg/mL,respectively,and the FGF-23 level increased to 896.35 pg/L.The differences between the data of these two groups of rats were statistically significant(p<0.05).The levels of 25(OH)VD3,1,25(OH)VD3 and Klotho in the high dose lentinan group increased to 71.24 ng/mL,4.85 ng/mL and 58.68 pg/mL,respectively,which were statistically higher than those in the vitamin D3 group and the low dose lentinan group.Whereas,the level of FGF-23 of the high dose lentinan group decreased to 552.23 pg/L,which was statistically lower than those in the vitamin D3 group and the low dose lentinan group(p<0.05).Lentinan polysaccharides acts on the vitamin D-FGF-23-Klotho axis,regulates the axis factors,and increases the vitamin D content to improve osteogenesis and protect the bones of the vitamin D deficient rats.