毛郁金根茎内生真菌的分离纯化及其抗心肌细胞缺氧/复氧损伤的保护作用

Isolation and purification of endophytic fungi from the rhizomes of Curcuma aromatica Salisb.and their protective effect against hypoxia/reoxygenation injury of cardiomyocytes

目的:探究毛郁金根茎内生真菌的种类及其体外抗心肌细胞缺氧/复氧(H/R)损伤的活性。方法:采用组织块贴壁法提取、分离、纯化毛郁金根茎内生真菌,PDB发酵培养,MTT法对粗提物进行抗心肌细胞H9C2 H/R损伤的保护作用筛选。结果:本研究从毛郁金根茎中分离并鉴定出23株内生真菌,其中17株鉴定到种,6株鉴定到属;11株内生真菌的粗提物在一定浓度范围内无明显细胞毒性;11株无明显细胞毒性的内生真菌中有2株对H9C2心肌细胞H/R损伤具有保护作用(P<0.05)。结论:本研究分离的内生真菌的活性结果与毛郁金根茎体外抗H/R作用类似,为进一步从该植物内生真菌中寻找抗心肌损伤保护作用的化学成分提供了可行性依据。

Objective:To explore the species of endophytic fungi from the rhizomes of Curcuma aromatica Salisb.(C.aromatica),and their anti-hypoxia/reoxygenation(H/R)injury of cardiomyocytes in vitro.Methods:The endophytic fungi in the rhizomes were extracted and purified by tissue block attachment method.The purified endophytic fungi were cultured by PDB medium,and the protective effect of crude extracts against H9C2 H/R injury of cardiomyocytes was screened by MTT method.Results:In this study,23 endophytic fungi were isolated and identified from the rhizomes of C.aromatica.Among them,17 strains were identified to species and 6 strains to genera;the crude extracts of 11 strains of endophytic fungi showed no obvious cytotoxicity within a certain concentration range.Two of the 11 endophytic fungi without obvious cytotoxicity showed protective effect on anti-myocardial H/R injury in H9C2 cells(P<0.05).Conclusion:The activity results of the endophytic fungi isolated in this study are similar to the anti-H/R effect of the rhizomes of C.aromatica in vitro,which provides a feasible basis for further searching for chemical components with protective effect against myocardial injury from the endophytic fungi.