4Boersma Y L, Droge M J, Quax W J. Selection strategies for improved biocatalysts [J].The FEBS journal,2007,274(9):2181- 2195.
5Arnold F H. Directed evolution: creating biocatalysts for the future[J].Chemical Engineering Science,1996,51(23):5091-5102.
6Kuchner O, Arnold F H. Directed evolution of enzyme catalysts [J].Trends Biotechnol,1997,15(12):523-530.
7Chen K Q, Arnold F H. Enzyme engineering for nonaqueous solvents: random mutagenesis to enhance activity of subtiiisin E in polar organic media[J].Nature Biotechnology,1991(9):1073- 1077.
8Leung D W, Chen E, Goeddel D V. A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction[J].Technique,1989(1):11-15.
9Stemmer W P. Rapid evolution of a protein in vitro by DNA shuffling [J].Nature, 1994,370:389-391.
10Shao Z, Zhao H, Giver L, et al. Random-priming in vitro recombination: an effective tool for directed evolution [J]. Nucleic Acids Res,1998,26(2):681-683.
