染色体构象捕获技术及其衍生高通量技术发展与展望

Development and Prospect of Chromosome Conformation Capture Technology and Its Derivative High-throughput Technology

细胞核内染色质的三维空间组织功能性地调控基因组的表达。染色质动态的三维组织研究是表观遗传学的一个重要研究领域,而染色体构象捕获(chromosome conformation capture,3C)技术是研究三维基因组结构与功能的重要技术手段。随着该技术在表观遗传学研究领域的广泛应用,3C衍生出的相关技术有了快速的发展。本文将对近年来发展起来的染色体相互作用的相关研究技术及其发展历程和基本原理等作介绍与展望,为三维基因组研究提供技术参考,为未来相关技术的发展提供新思路。

The three-dimensional tissue of chromatin in the nucleus functionally regulates the expression of the genome.The study of dynamic three-dimensional tissue of chromatin is an important research field of epigenetics,and chromosome conformation capture(3C)is an important technical measure to study the structure and function of three-dimensional genome.With the wide application of this technology in the field of epigenetics,the related techniques derived from 3C have developed rapidly.This paper will introduce and prospect the related technologies,development process and basic principles of chromosome interaction developed in recent years,so as to provide technical reference for three-dimensional genome research and provide new ideas for the development of related technologies in the future.