柳枝稷长链非编码RNA PvLINC090588的功能研究

Functional Study of Switchgrass Long Noncoding RNA PvLINC090588

长链非编码RNA(long noncoding RNA,lncRNA)是一类长度大于200个核苷酸、无蛋白编码能力或蛋白编码能力较低的功能性RNA分子,可通过调控基因的表达影响植物的生长发育和对非生物胁迫的适应性。柳枝稷(Panicum virgatum L.)是一种抗旱性较强的禾本科(Poaceae)C_(4)生物能源模式作物。本研究从柳枝稷中克隆了1个新预测的lncRNA,将其命名为PvLINC090588。对该基因的表达模式分析显示:20%聚乙二醇、150 mmol/L氯化钠和50μmol/L脱落酸可诱导其表达,100μmol/L赤霉素抑制其表达,表明PvLINC090588的表达受多种非生物胁迫的诱导。过表达PvLINC090588转基因拟南芥(Arabidopsis thaliana)在100 mmol/L氯化钠、300 mmol/L甘露醇处理下其根长显著(P<0.05)大于野生型的,显示过表达株系的根系在胁迫下生长能力更强。与野生型相比,转基因株系在干旱和250 mmol/L氯化钠处理后其丙二醛含量降低,可溶性糖和脯氨酸含量显著升高(P<0.05),表明过表达株系在胁迫下的细胞损伤程度低于野生型的,但渗透调节物质含量高于野生型的。以上结果表明,过表达长链非编码RNA PvLINC090588在一定程度上提高了拟南芥的抗旱和抗盐能力,为柳枝稷及其他禾本科作物抗旱和抗盐的机制解析提供重要参考。

Long noncoding RNA(lncRNA),a length of more than 200 nucleotides,is a class of functional RNA with no or low protein-coding capacity,which can affect plant development and the adaptability to abiotic stress by regulating gene expression.Switchgrass(Panicum virgatum L.)is a Poaceae C_(4) bioenergy model crop with strong drought resistance.In this study,a newly predicted lncRNA named PvLINC090588 was cloned from switchgrass.Expression pattern analysis showed that 20%PEG,150 mmol/L NaCl and 50μmol/L ABA induced its expression,and 100μmol/L GA inhibited its expression,indicating that the expression of Pv-LINC090588 was induced by various abiotic stresses.Overexpression of PvLINC090588 in Arabidopsis thaliana significantly increased root length under 100 mmol/L NaCl and 300 mmol/L mannitol treatments(P<0.05),indicating that the roots of the overexpressed lines had stronger roots growth ability under stress.Compared with wild type A.thaliana,malondialdehyde content of transgenic lines were decreased,while the soluble sugar and proline contents were significantly increased(P<0.05)under the drought and 250 mmol/L NaCl treatment,indicating that the cell damage of overexpressed lines were more severe,but the content of osmotic adjustment substances was higher than that of wild type A.thaliana.These results showed that the overexpression of PvLINC090588 enhanced drought and salt resistance of A.thaliana,and provided an important reference for the mechanism analysis of drought and salt resistance in switchgrass and other crops.