摘要
绿原酸(chlorogenic acid,CGA)作为一种重要的次生代谢物,在医药、食品、化工等领域广泛应用。本实验室前期研究发现一株产绿原酸的解淀粉芽孢杆菌(Bacillus Amyloliquefaciens)B17,绿原酸产量为1.746 mg/L。为改善该菌株产绿原酸的能力,利用单因素设计优化培养基的培养成分和发酵条件。获得了最佳培养基组分为:蔗糖40 g/L,NH_(4)Cl 5.0 g/L,KH_(2)PO_(4)1.0 g/L,NaCl 3.0 g/L,CuSO_(4)0.025 g/L,FeSO_(4)0.1 g/L,L-苯丙氨酸2 g/L,对羟基苯甲酸10 g/L;并且利用正交设计对发酵条件进行了优化,获得的发酵条件为:温度40℃,pH值7.0,装液量40 mL/250 mL和转速220 r/min。在该条件下,菌株B17产量为1.28 g/L,较优化前产量提高了733.1倍。上述研究结果表明,发酵优化显著提高了菌株B17生产绿原酸的能力,这为后期开发绿原酸在种植、养殖及重金属镉污染等领域的应用提供了原料来源。
Chlorogenic acid(CGA)is widely used as an important secondary metabolite in the fields of medicine,food,and chemical industry.A bacterium strain Bacillus amyloliquefaciens B17 was preserved in our laboratory with a chlorogenic acid production of 1.746 mg/L.A design of single-factor experiment was used to optimize the medium composition and fermentation conditions to improve the production of chlorogenic acid.The optimum medium components were obtained:sucrose 40 g/L,NH_(4)Cl 5.0 g/L,KH_(2)PO_(4)1.0 g/L,NaCl 3.0 g/L,CuSO_(4)0.025 g/L,FeSO_(4)0.1 g/L,L-phenylalanine acid 2 g/L,p-hydroxybenzoic acid 10 g/L.Orthogonal design to optimize the fermentation conditions,the fermentation conditions are:temperature 40℃,pH 7.0,liquid volume 40mL/250 mL and rotational speed 220 r/min.Under this condition,the yield of the strain Bacillus Amyloliquefaciens B17 was 1.28 g/L,which was 733.1 times higher than that before optimization.The above results indicate that the fermentation optimization significantly improved the ability of strain B17 to produce chlorogenic acid,which provides a raw material basis for the later development of CGA application in the fields of planting,breeding and removal of heavy metal cadmium pollution.
作者
王晓
龚彬婷
昝青佳
李柯
李有志
樊宪伟
Wang Xiao;Gong Binting;Zan Qingjia;Li Ke;Li Youzhi;Fan Xianwei(State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources,College of Life Science and Technology,Guangxi University,Nanning,530004)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2021年第11期3492-3499,共8页
Genomics and Applied Biology
基金
科技基础性工作专项项目(2014FY120100)
广西科技计划项目(桂科AB17292064)共同资助
关键词
解淀粉芽孢杆菌B17
绿原酸
单因素试验
正交试验
Bacillus amyloliquefaciens B17
Chlorogenic acid
Single factor test
Orthogonal test