两株单核体金针菇全基因组tRNA分析

Analysis of tRNA in Two Monokaryotic Genomes of Flammulina velutipes

金针菇(Flammulina velutipes)是一种重要的商业化栽培食用菌,具有较高的营养和药用价值。本研究基于全基因组测序技术,预测了两个金针菇单核菌株W23和L11基因组中的tRNA种类与数量。金针菇W23菌株基因组包含1866个Scaffolds,共鉴定到204个tRNA基因,其中有25个可能的假基因和3个未知的异型结构。金针菇L11菌株基因组包含1858个Scaffolds,共鉴定到194个tRNA基因,其中有27个可能的假基因,5个未知的异性结构。金针菇tRNA最大长度达到244 bp,最小长度仅为58 bp,总长度占基因组比例约为0.05%。两个金针菇单核菌株均没有鉴定到被称为人体第21种氨基酸的硒代半胱氨酸和可能的抑制性基因。本研究对金针菇的基因组tRNA编码基因的种类进行了分析,将有助于进一步研究其分子功能以及金针菇的系统进化。

Flammulina velutipes is an important commercial cultivation of edible fungi,with high nutritional and medicinal value.Based on the genomes of two monokaryotic strains,we analyzed the tRNAs in the two F.velutipes.In the 1866 scaffolds of W23 genome,204 tRNA genes were identified,including 25 possible pseudogenes,3 unknown isoforms.In the 1856 scaffolds of L11 genome,194 tRNA genes were identified,including 27 possible pseudogenes,5 unknown isoforms.The max tRNA length was 244 bp,and the min one was 58 bp.Total length was about 0.05%of the genome.There was no selenocysteine and possible suppressor tRNAs in both monokaryotic strains.Analysis of the tRNA coding genes will be helpful to further study the molecular function and phylogeny of F.velutipes evolution.