饱和与不饱和脂肪酸对巨噬细胞炎症状态的影响

Effects of Saturated and Unsaturated Fatty Acids on Macrophage Inflammatory Status

选取8种脂肪酸,分别刺激体外培养的小鼠巨噬细胞,再分析炎症标志基因表达的变化,旨在揭示各种脂肪酸对巨噬细胞炎症状态的影响。结果表明,4种饱和脂肪酸棕榈酸(PA)、硬脂酸(SA)、二十二烷酸(BA)和二十四烷酸(LA)均诱发巨噬细胞炎症标志基因TNFα表达上调,细胞呈现炎症状态,PA最显著;4种不饱和脂肪酸棕榈油酸(PO)、花生四烯酸(ARA)、二十碳五烯酸(EPA)和二十二碳六烯酸(DHA)均诱发巨噬细胞抗炎症标志基因IL10表达上调,细胞呈现抗炎症状态,DHA最显著;此外,在PA诱导的炎症反应后,添加不饱和脂肪酸能够缓解炎症状态,ARA最显著,DHA次之。Western blot分析发现,PA、LA、ARA和DHA都是通过NF-κB信号通路影响巨噬细胞炎症状态。综上,本研究发现饱和脂肪酸PA、SA、BA和LA能诱发巨噬细胞炎症反应,而不饱和脂肪酸PO、ARA、EPA和DHA能缓解巨噬细胞炎症反应,并且ARA表现最佳。

Eight kinds of fatty acids were added into bone marrow derived mouse microphages,respectively,then the mRNA abundance of the inflammation marker genes was analyzed to unveil the effects of fatty acids on macrophages inflammation.Results showed that saturated fatty acids,Palmitate acid(PA),Stearic acid(SA),Behenic acid(BA)and Lignoceric acid(LA),induced upregulation of TNFα,which indicated the macrophage became pro-inflammatory,and the effects of PA were highest.Unsaturated fatty acids,Palmitoleate acid(PO),Arachidonic acid(ARA),Eicosapentaenoic acid(EPA)and Docosahexaenoic acid(DHA),induced upregulation of IL10,which indicated the macrophage became anti-inflammatory,and the effects of DHA were the highest.Furthermore,added unsaturated fatty acids could reduce inflammatory states induced by PA,the effects of ARA were the most significant,followed by DHA.Western blot showed that the effects of PA,LA,ARA and DHA on macrophages inflammation were through NF-κB signaling pathway.In summary,saturated fatty acids PA,SA,BA and LA induce macrophages pro-inflammatory,while unsaturated fatty acid PO,ARA,EPA and DHA can alleviated macrophages inflammatory,with the best effects of ARA.