豹纹鳃棘鲈幼鱼黑色素合成相关基因的表达分析

Expression Analysis of Melanin Synthesis-Related Genes in Juvenile Plectropomus leopardus

体色作为鱼类形态特征之一,其丰富的变化规律一直是人们关注的焦点。在人工养殖过程中,豹纹鳃棘鲈(Plectropomus leopardus)幼鱼体色会出现由红变黑的现象,这是研究其体色发育的重要材料。本研究基于豹纹鳃棘鲈基因组数据,获得了参与黑色素合成相关基因的ORF序列(EDNRB,MC1R,SOX10,TYRL,TYRP1和WNT5A)。随后,对这些基因的氨基酸序列进行分析,并通过RT-qPCR检测它们在红黑体色转换过程中的表达差异。结果表明,与其他硬骨鱼类相比,豹纹鳃棘鲈的EDNRB,MC1R,SOX10,TYRP1,WNT5A基因分化程度较低,而TYRL分化程度较高。豹纹鳃棘鲈黑色皮肤幼鱼与红色皮肤幼鱼相比,TYRL和TYRP1基因表达显著性上调,而MC1R,EDNRB,WNT5A,SOX10基因的表达在两种肤色个体中并未呈现出显著差异。本研究结果表明,TYRL和TYRP1基因可能参与豹纹鳃棘鲈幼鱼体色由红变黑的调控。

Body color is one of the morphological characteristics of fish.The rich change rule have alway been the focus of peoples attention.In the process of artificial culture,the body color of Plectropomus leopardus juveniles will change from red to black,which is an important material to study the development of body color.This research based on genomic data of P.leopardus and obtained the sequences of genes involved in melena synthesis(EDNRB,MC1R,SOX10,TYRL,TYRP1 and WNT5 A).Then,the amino acid sequences encoded by genes were analysed,and their expression differences in the process of the red black body were detected by RT-qPCR.The results showed that EDNRB,MC1 R,SOX10,TYRP1 and WNT5 A genes of P.leopardus were less differentiated than other teleost’s,while TYRL genes were more differentiated.The expressions of TYRL and TYRP1 were found to be significantly up-regulated in black-skinned juveniles of P.leopardus compared to red-skinned juveniles,while there were no significant difference in gene expression of EDNRB,MC1 R,SOX10 and WNT5 A.The results showed that TYRL and TYRP1 genes may be involved in the regulation of body color of P.leopardus from red to black.