长期低氧胁迫下斑马鱼的卵巢转录组特征分析

Analysis of Zebrafish Ovary Transcriptome under Long-term Hypoxia Stress

为探明低氧胁迫对雌性性腺发育的影响,本研究针对常氧与长期低氧胁迫下的斑马鱼卵巢组织进行转录组测序,研究长期低氧胁迫与常氧条件下斑马鱼卵巢组织的转录组和基因表达谱差异。低氧与常氧卵巢差异表达显著的基因总数为675个,其中381个基因在低氧卵巢中显著下调,294个基因在低氧卵巢中表达量显著上调。对差异表达显著的基因进行GO聚类,主要富集在胰岛素样生长因子结合、细胞因子活性、细胞黏附分子结合等7个GO项目上。对低氧与常氧斑马鱼卵巢进行组织切片观察后发现,与常氧斑马鱼的卵巢相比,长期低氧胁迫后的斑马鱼卵巢的卵母细胞数目更少且发育较慢。由此推测:长期低氧胁迫可影响卵巢发育,从而影响卵母细胞的发育成熟。本研究结果为深入了解低氧环境对斑马鱼生长发育的影响提供了基础。

In order to explore the possible influences of hypoxia on female gonad development,this study performed transcriptome sequencing on zebrafish(Danio rerio)ovarian tissue under normoxia and chronic hypoxia stress to study the transcriptome of zebrafish ovary tissue under long-term hypoxic stress and normoxic conditions and gene expression profile differences.The total number of significantly differentially expressed genes in hypoxic and normoxic ovaries was 675.Among the differentially expressed genes,381 and 294 genes showed significantly down-regulated and up-regulated expressions,respectively.GO enrichment analysis based on the differentially expressed genes were enriched in 7 GO terms including insulin-like growth factor binding,cytokine activity and cell adhesion molecule binding.Observation of tissue sections of hypoxic and normoxic zebrafish ovary revealed that compared with normoxic zebrafish ovary,the number of oocytes in zebrafish ovary after long-term hypoxia stress was less and the development was slower.It is speculated that the long-term hypoxic stress affects ovarian development and thus affects the maturation of oocytes.The results of this study provide a basis for a deeper understanding of the effects of hypoxic environment on the growth and development of zebrafish.