两种不同化感潜力水稻CYP73A38基因及其启动子的克隆分析

Cloning Analysis of CYP73A38 Gene and Its Promoter in Two Different Allelopathic Potential of Rice

水稻化感作用指水稻通过向环境中释放次生代谢产物来影响周围植物的生长。本研究以国际公认的化感水稻PI312777和非化感水稻Lemont为材料,克隆肉桂酸-4-羟化酶(C4H)编码基因CYP73A38的cDNA序列进行对比分析,发现两种水稻在该基因的序列差异极小,存在6个位点差异。为了进一步揭示两种水稻化感潜力不同的原因,对CYP73A38启动子进行了克隆分析,获得CYP73A38基因启动子序列,该启动子序列包含CAAT-box、TATA-box、光响应元件、赤霉素响应元件、生长素响应元件、低温响应元件等多个顺式作用元件及MYB类转录因子结合位点,且差异位点均不在顺式作用元件上,由此推测两种水稻化感潜力的差异与基因结构没有相关性。不同化感潜力水稻CYP73A38基因及其启动子的克隆与序列分析,将为进一步研究CYP73A38基因的表达调控及其启动子功能分析提供参考,为加快水稻化感抗性品种的选育进程提供依据。

Rice alle lopathy is mainly responsible for the growth of around plant by releasing secondary metabolites into the environment.In this study,allelopathic rice PI312777 and non-allelopathic rice Lemont were used as materials,cloned the cinnamic acid-4-hydroxylase(C4H)coding gene CYP73A38 full length of sequence cDNA and comparatived difference.The differences in the sequence of this gene in allelopathic and non-allelic rice were slight.A total of 6 nucleotide variations were found in the ORFs.In order to research the reason of two rice with different allelopathic potential,the study cloned the promoter sequence of CYP73A38.The promoter sequence contained multiple cis-acting elements,such as CAAT-box,TATA-box,light responsive element,gibberellins responsive element,auxin responsive element,low-temperature responsive element,MYB recognition site,and others.And all variation sites are not in the cis-acting elements.Thus speculate that the differences of two allelopathy potential rice do es not correlate with the genetic structure.Cloning and characterization of the CYP73A38 gene and its promoter will provide basis for the further study of regulation and functional analysis of CYP73A38 gene in rice and the breeding process of allelopathic resistant rice varieties.